ALDOC ELISA 试剂盒
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- 抗原 See all ALDOC ELISA试剂盒
- ALDOC (Aldolase C, Fructose-Bisphosphate (ALDOC))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Aldolase C, Fructose Bisphosphate (ALDOC). No significant cross-reactivity or interference between Aldolase C, Fructose Bisphosphate (ALDOC) and analogues was observed.
- 灵敏度
- 0.055 ng/mL
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Aldolase C, Fructose-Bisphosphate (ALDOC) ELISA Kit
ALDOC 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Aldolase C, Fructose-Bisphosphate (ALDOC) ELISA KitALDOC 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Aldolase C, Fructose-Bisphosphate (ALDOC) ELISA KitALDOC 适用: 大鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aldolase C, Fructose Bisphosphate (ALDOC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aldolase C, Fructose Bisphosphate (ALDOC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aldolase C, Fructose Bisphosphate (ALDOC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aldolase C, Fructose Bisphosphate (ALDOC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aldolase C, Fructose Bisphosphate (ALDOC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aldolase C, Fructose Bisphosphate (ALDOC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all ALDOC ELISA试剂盒
- ALDOC (Aldolase C, Fructose-Bisphosphate (ALDOC))
- 别名
- Aldolase C, Fructose Bisphosphate (ALDOC 产品)
- 别名
- ALDC ELISA Kit, MGC69434 ELISA Kit, ALDOC ELISA Kit, aldoc ELISA Kit, AI847350 ELISA Kit, AU040929 ELISA Kit, Aldo3 ELISA Kit, Scrg2 ELISA Kit, ALDCAA ELISA Kit, F16dip7 ELISA Kit, RATALDCAA ELISA Kit, aldocl ELISA Kit, wu:fj56h04 ELISA Kit, zgc:112357 ELISA Kit, QccE-21970 ELISA Kit, aldolase, fructose-bisphosphate C ELISA Kit, aldolase, fructose-bisphosphate C L homeolog ELISA Kit, aldolase C, fructose-bisphosphate, b ELISA Kit, aldolase C, fructose-bisphosphate ELISA Kit, aldolase A, fructose-bisphosphate ELISA Kit, aldolase C, fructose-bisphosphate, a ELISA Kit, Fructose-bisphosphate aldolase C ELISA Kit, ALDOC ELISA Kit, aldoc.L ELISA Kit, aldoc ELISA Kit, aldocb ELISA Kit, Aldoc ELISA Kit, ALDOA ELISA Kit, aldoca ELISA Kit
- 背景
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Gene Name: Aldolase C, Fructose Bisphosphate
Gene Aliases: ALDO-C, ALDC, ALD-C, Fructose-bisphosphate aldolase C, Brain-type aldolase
- 基因ID
- 230
- UniProt
- P09972
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