ARL15 ELISA 试剂盒
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- 抗原 See all ARL15 ELISA试剂盒
- ARL15 (ADP-Ribosylation Factor-Like 15 (ARL15))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 78 pg/mL - 5000 pg/mL
- 最低检测浓度
- 78 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of ADP Ribosylation Factor Like Protein 15 (ARL15). No significant cross-reactivity or interference between ADP Ribosylation Factor Like Protein 15 (ARL15) and analogues was observed.
- 灵敏度
- 29 pg/mL
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ADP-Ribosylation Factor-Like 15 (ARL15) ELISA Kit
ARL15 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADP Ribosylation Factor Like Protein 15 (ARL15). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ADP Ribosylation Factor Like Protein 15 (ARL15). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ADP Ribosylation Factor Like Protein 15 (ARL15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ADP Ribosylation Factor Like Protein 15 (ARL15) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ADP Ribosylation Factor Like Protein 15 (ARL15) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ADP Ribosylation Factor Like Protein 15 (ARL15) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all ARL15 ELISA试剂盒
- ARL15 (ADP-Ribosylation Factor-Like 15 (ARL15))
- 别名
- ADP Ribosylation Factor Like Protein 15 (ARL15 产品)
- 别名
- ARFRP2 ELISA Kit, A430036I03 ELISA Kit, Arfrp2 ELISA Kit, C230032K13Rik ELISA Kit, MGC84452 ELISA Kit, arfrp2 ELISA Kit, MGC89785 ELISA Kit, DKFZp469A1522 ELISA Kit, ADP ribosylation factor like GTPase 15 ELISA Kit, ADP-ribosylation factor-like 15 ELISA Kit, ADP-ribosylation factor like GTPase 15 ELISA Kit, ADP ribosylation factor like GTPase 15 L homeolog ELISA Kit, ARL15 ELISA Kit, Arl15 ELISA Kit, arl15.L ELISA Kit, arl15 ELISA Kit
- 背景
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Gene Name: ADP Ribosylation Factor Like Protein 15
Gene Aliases: ARFRP2, ADP-Ribosylation Factor Related Protein 2
- 基因ID
- 54622
- UniProt
- Q9NXU5
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