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Exosome Purification and Analysis Kit (Cell culture)

FACS 适用: 人 Fluorometric Cell Culture Cells
产品编号 ABIN5633279
发货至: 中国
  • 适用
    检测方法
    Fluorometric
    标记
    PE
    应用范围
    Flow Cytometry (FACS)
    原理
    Exosome purification and FACS analysis kit of exosomes derived from cell culture
    品牌
    ExoStep™
    样品类型
    Cell Culture Cells
    产品特性
    The kit is a simple immunobead assay for isolation/detection of exosome, using a bead-bound anti-CD63 capture antibody and a fluorochrome conjugated anti-CD9 detection antibody. The kit provides reproducible results and can be run in parallel to exosome immunophenotyping.
    组件
    CD63+ capture beads Anti-CD9 biotin Streptavidin-Phycoerythrin (PE) Assay Buffer 10X
    试剂未包括
    • Pre-enriched exosomes by ultra-centrifugation. • Magnetic Rack, MagneSphere(R) Mag. Sep. Stand 12-hole, 12x75mm (PROMEGA, Ref Z5343). • 12x75 mm Polystyrene Round Bottom Tubes (cytometer
  • tubes
  • ). • Sterile syringe filter with a 0.22 μm pore. (EMD Millipore, Ref SLGSV255F) • Syringe of adequate volume
  • 应用备注
    Isolate and purify exosomes by FACS with reproducible results. You can calculate the concentration of protein easily and without other protein contaminations. It allows also immunophenotyping of the captured exosomes
    说明

    Very reproducible and faster than WB or TME.3 hours for enrichement the sample+ Overnight Incubation+ 1 hour of protocol

    样本量
    50 μL
    实验时间
    4 h
    试剂准备

    Reagents ready to use.

    限制
    仅限研究用
  • 缓冲液
    The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store in the dark at 2-8 °C
  • 背景
    Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB)1, with the plasma membrane. They are thought to provide a means of intercellular communication and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases.
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