Biotin ELISA 试剂盒
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- 抗原 See all Biotin products
- Biotin
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适用
- Various Species
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 32 pg/mL - 20000 pg/mL
- 最低检测浓度
- 32 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
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This assay has high sensitivity and excellent specificity for detection of Vitamin B7 (VB7).
No significant cross-reactivity or interference between Vitamin B7 (VB7) and analogues was observed. - 灵敏度
- 13 pg/mL
- 组件
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- Pre-coated, ready to use 96-well strip plate
- Plate sealer for 96 wells
- Reference Standard
- Standard Diluent
- Detection Reagent A
- Assay Diluent A
- TMB Substrate
- Stop Solution
- Wash Buffer (30 x concentrate)
- Instruction manual
- Featured
- Discover our best selling Biotin ELISA Kit
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Biotin ELISA Kit
适用: Various Species Colorimetric Competition ELISA 32 pg/mL - 20000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 样本量
- 50 μL
- 实验时间
- 2 h
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards,
- Add 50μL standard or sample to each well.
Then add 50μL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37 °C, - Aspirate and wash 5 times,
- Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
- Add 50μL Stop Solution. Read at 450 nm immediately.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vitamin B7 (VB7) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vitamin B7 (VB7) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
- 限制
- 仅限研究用
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- 注意事项
- The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
- 注意事项
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. - 储存条件
- 4 °C/-20 °C
- 储存方法
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- For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
- For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit. - For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.
- 有效期
- 6 months
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- 抗原 See all Biotin products
- Biotin
- 别名
- Vitamin B7 (VB7) (Biotin 产品)
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