TMSB4X ELISA 试剂盒
-
- 抗原 See all TMSB4X ELISA试剂盒
- TMSB4X (Thymosin beta-4 (TMSB4X))
-
适用
- 人, 大鼠, 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 0.1-1.000 ng/mL
- 最低检测浓度
- 0.1 ng/mL
- 应用范围
- ELISA
- 原理
- Human/Mouse/Rat Thymosin beta-4 EIA Kit optimized for serum, plasma and cell culture media. Competition-based ELISA on a 96-well strip plate.
- 样品类型
- Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This EIA kit is designed to detect human, mouse, and rat Thymosin-b4
- 灵敏度
- 1.1 ng/mL
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
- Featured
- Discover our best selling TMSB4X ELISA Kit
- Top Product
- Discover our top product TMSB4X ELISA Kit
-
Thymosin beta-4 (TMSB4X) ELISA Kit
TMSB4X 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Thymosin beta-4 (TMSB4X) ELISA KitTMSB4X 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Thymosin beta-4 (TMSB4X) ELISA KitTMSB4X 适用: 人 Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Plasma, Serum
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed.
- Add 100 μL detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4 °C.
- Add 100 μL standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 μL prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 μL TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL Stop Solution to each well.
- Read plate at 450 nm immediately.
- 试剂准备
-
Recommended Dilution for serum and plasma samplesHuman: 2x / Mouse: 2x / Rat: 2x
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
- 有效期
- 6 months
-
- 抗原 See all TMSB4X ELISA试剂盒
- TMSB4X (Thymosin beta-4 (TMSB4X))
- 别名
- Thymosin beta-4 (TMSB4X 产品)
- 别名
- FX ELISA Kit, PTMB4 ELISA Kit, TB4X ELISA Kit, TMSB4 ELISA Kit, Ptmb4 ELISA Kit, Tb4 ELISA Kit, tmsb4 ELISA Kit, TMSB4X ELISA Kit, thymosin beta 4, X-linked ELISA Kit, thymosin, beta 4, X chromosome ELISA Kit, thymosin beta 4, X-linked L homeolog ELISA Kit, thymosin, beta 4-like ELISA Kit, thymosin beta 4 pseudogene ELISA Kit, TMSB4X ELISA Kit, Tmsb4x ELISA Kit, tmsb4x.L ELISA Kit, TMSB4 ELISA Kit, LOC100339394 ELISA Kit, LOC100860922 ELISA Kit
- 基因ID
- 7114
- UniProt
- P62328
- 途径
- Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Maintenance of Protein Location, Smooth Muscle Cell Migration
-
