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RICTOR ELISA 试剂盒

RICTOR 适用: 人, 小鼠 pThr1135 Colorimetric Cell ELISA Adherent Cell Culture
产品编号 ABIN5526752
发货至: 中国
  • 抗原 See all RICTOR ELISA试剂盒
    RICTOR (RPTOR Independent Companion of mTOR, Complex 2 (RICTOR))
    抗原表位
    pThr1135, total
    适用
    人, 小鼠
    检测方法
    Colorimetric
    实验类型
    Cell ELISA
    应用范围
    ELISA
    原理
    Cell-Based ELISA Kit. This assay semi-quantitatively measures Rictor phosphorylated at Threonine-1135 as well as total Rictor in adherent cell lines.
    样品类型
    Adherent Cell Culture
    Analytical Method
    Semi-Quantitative
    特异性
    This ELISA kit recognizes Human and Mouse Rictor phosphorylated at site Threonine-1135 as well as total Rictor.
    产品特性
    • Rapidly measure phosphorylated protein in adherent cell lines
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • No sample lysis is needed
    • Compatible with a standard ELISA plate reader
    组件
    • Uncoated 96-well Strip Microplate
    • Wash Buffers
    • Fixing Solution
    • Quenching Buffer
    • Blocking Buffer
    • Anti-phospho antibody
    • Anti-pan antibody
    • HRP-Conjugated Secondary Antibody
    • TMB One-Step Substrate
    • Stop Solution
    试剂未包括
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product RICTOR ELISA Kit
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    有效期
    6 months
  • 抗原 See all RICTOR ELISA试剂盒
    RICTOR (RPTOR Independent Companion of mTOR, Complex 2 (RICTOR))
    别名
    Rictor (RICTOR 产品)
    别名
    PIA ELISA Kit, mAVO3 ELISA Kit, 4921505C17Rik ELISA Kit, 6030405M08Rik ELISA Kit, AVO3 ELISA Kit, AW492497 ELISA Kit, D530039E11Rik ELISA Kit, Fyb ELISA Kit, RPTOR independent companion of MTOR complex 2 ELISA Kit, RPTOR independent companion of MTOR, complex 2 ELISA Kit, RICTOR ELISA Kit, Rictor ELISA Kit
    基因ID
    253260
    UniProt
    Q6R327, Q6QI06
    途径
    Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, CXCR4-mediated Signaling Events
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