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PKMYT1 ELISA 试剂盒

PKMYT1 适用: 人 pThr495 Colorimetric Cell ELISA Adherent Cell Culture
产品编号 ABIN5526747
发货至: 中国
  • 抗原 See all PKMYT1 ELISA试剂盒
    PKMYT1 (Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1))
    抗原表位
    pThr495
    适用
    检测方法
    Colorimetric
    实验类型
    Cell ELISA
    应用范围
    ELISA
    原理
    Cell-Based ELISA Kit. This assay semi-quantitatively measures PKMYT1 phosphorylated at Threonine-495 as well as total PKMYT1 in adherent cell lines.
    样品类型
    Adherent Cell Culture
    Analytical Method
    Semi-Quantitative
    特异性
    This ELISA kit recognizes Human PKMYT1 phosphorylated at site Threonine-495 as well as total PKMYT1.
    产品特性
    • Rapidly measure phosphorylated protein in adherent cell lines
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • No sample lysis is needed
    • Compatible with a standard ELISA plate reader
    组件
    • Uncoated 96-well Strip Microplate
    • Wash Buffers
    • Fixing Solution
    • Quenching Buffer
    • Blocking Buffer
    • Anti-phospho antibody
    • Anti-pan antibody
    • HRP-Conjugated Secondary Antibody
    • TMB One-Step Substrate
    • Stop Solution
    试剂未包括
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product PKMYT1 ELISA Kit
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    有效期
    6 months
  • 抗原 See all PKMYT1 ELISA试剂盒
    PKMYT1 (Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1))
    别名
    PKMYT1 (PKMYT1 产品)
    别名
    MYT1 ELISA Kit, 6230424P17 ELISA Kit, AW209059 ELISA Kit, Myt1 ELISA Kit, RGD1305434 ELISA Kit, myt1 ELISA Kit, protein kinase, membrane associated tyrosine/threonine 1 ELISA Kit, protein kinase, membrane associated tyrosine/threonine 1 S homeolog ELISA Kit, PKMYT1 ELISA Kit, pkmyt1 ELISA Kit, Pkmyt1 ELISA Kit, pkmyt1.S ELISA Kit
    基因ID
    9088
    UniProt
    Q99640
    途径
    Mitotic G1-G1/S Phases, M Phase
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