DDR1 ELISA 试剂盒
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- 抗原 See all DDR1 ELISA试剂盒
- DDR1 (Discoidin Domain Receptor tyrosine Kinase 1 (DDR1))
- 抗原表位
- pTyr792
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Phospho-DDR1 (Tyr792) ELISA Kit. This assay semi-quantitatively measures DDR1 phosphorylated at Tyrosine-792 in cell lysate samples.
- 样品类型
- Cell Culture Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- This ELISA kit recognizes Human DDR1 phosphorylated at site Tyrosine-792.
- 产品特性
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Featured
- Discover our best selling DDR1 ELISA Kit
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Discoidin Domain Receptor tyrosine Kinase 1 (DDR1) ELISA Kit
DDR1 适用: 人 pTyr792 Colorimetric Sandwich ELISA Cell Culture Lysate
Discoidin Domain Receptor tyrosine Kinase 1 (DDR1) ELISA KitDDR1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Discoidin Domain Receptor tyrosine Kinase 1 (DDR1) ELISA KitDDR1 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all DDR1 ELISA试剂盒
- DDR1 (Discoidin Domain Receptor tyrosine Kinase 1 (DDR1))
- 别名
- DDR1 (DDR1 产品)
- 别名
- CG11573 ELISA Kit, CG13984 ELISA Kit, CG13985 ELISA Kit, CG33531 ELISA Kit, CG9488 ELISA Kit, CG9490 ELISA Kit, CT33540 ELISA Kit, DDR ELISA Kit, Dmel\\CG33531 ELISA Kit, Dmel_CG13984 ELISA Kit, 6030432F18 ELISA Kit, AI323681 ELISA Kit, CD167a ELISA Kit, Cak ELISA Kit, Nep ELISA Kit, PTK3A ELISA Kit, CAK ELISA Kit, CD167 ELISA Kit, EDDR1 ELISA Kit, HGK2 ELISA Kit, MCK10 ELISA Kit, NEP ELISA Kit, NTRK4 ELISA Kit, PTK3 ELISA Kit, RTK6 ELISA Kit, TRKE ELISA Kit, Drd1 ELISA Kit, PTK3D ELISA Kit, Discoidin domain receptor ELISA Kit, discoidin domain receptor family, member 1 ELISA Kit, discoidin domain receptor tyrosine kinase 1 ELISA Kit, Ddr ELISA Kit, Ddr1 ELISA Kit, DDR1 ELISA Kit
- 基因ID
- 780
- UniProt
- Q08345
- 途径
- RTK signaling, Smooth Muscle Cell Migration
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