EIF4E ELISA 试剂盒
EIF4E
适用: 人
pSer209
Colorimetric
Cell ELISA
Adherent Cell Culture
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- 抗原 See all EIF4E ELISA试剂盒
- EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))
- 抗原表位
- pSer209, total
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Cell ELISA
- 应用范围
- ELISA
- 原理
- Human Phospho-WNK1 (Thr60) and Total WNK1 Cell-Based ELISA Cell-Based ELISA Kit. This assay semi-quantitatively measures eIF-4E phosphorylated at Serine-209 as well as total eIF-4E in adherent cell lines.
- 样品类型
- Adherent Cell Culture
- Analytical Method
- Semi-Quantitative
- 特异性
- This ELISA kit recognizes Human eIF-4E phosphorylated at site Serine-209 as well as total eIF-4E.
- 产品特性
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- Rapidly measure phosphorylated protein in adherent cell lines
- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- No sample lysis is needed
- Compatible with a standard ELISA plate reader
- 组件
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- Uncoated 96-well Strip Microplate
- Wash Buffers
- Fixing Solution
- Quenching Buffer
- Blocking Buffer
- Anti-phospho antibody
- Anti-pan antibody
- HRP-Conjugated Secondary Antibody
- TMB One-Step Substrate
- Stop Solution
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Top Product
- Discover our top product EIF4E ELISA Kit
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all EIF4E ELISA试剂盒
- EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))
- 别名
- eIF-4E (EIF4E 产品)
- 别名
- AUTS19 ELISA Kit, CBP ELISA Kit, EIF4E1 ELISA Kit, EIF4EL1 ELISA Kit, EIF4F ELISA Kit, eif4e-1 ELISA Kit, zgc:86680 ELISA Kit, EG668879 ELISA Kit, Eif4e-ps ELISA Kit, If4e ELISA Kit, eIF-4E ELISA Kit, 0260/09 ELISA Kit, 0587/11 ELISA Kit, 0589/11 ELISA Kit, 0919/12 ELISA Kit, 1004/13 ELISA Kit, 2 ELISA Kit, 7238 ELISA Kit, CG4035 ELISA Kit, CT13384 ELISA Kit, CT39424 ELISA Kit, CT39426 ELISA Kit, D-eIF4E ELISA Kit, Dmel\\CG4035 ELISA Kit, Eif4E ELISA Kit, Eif4e ELISA Kit, IF4E ELISA Kit, dEif4e ELISA Kit, deIF-4E ELISA Kit, deIF4E ELISA Kit, eIF 4E ELISA Kit, eIF-4E1 ELISA Kit, eIF-4E2 ELISA Kit, eIF-4EII ELISA Kit, eIF-4e ELISA Kit, eIF4E ELISA Kit, eIF4E-1 ELISA Kit, eIF4E-1/2 ELISA Kit, eIF4E-2 ELISA Kit, eIF4E1 ELISA Kit, eIF4EI ELISA Kit, eIF4e ELISA Kit, eif-4E ELISA Kit, eif-4e ELISA Kit, eif4e ELISA Kit, l(3)07238 ELISA Kit, l(3)67Af ELISA Kit, l(3)S025007 ELISA Kit, l(3)S026009 ELISA Kit, l(3)S058711 ELISA Kit, l(3)S091912 ELISA Kit, cbp ELISA Kit, eif4f ELISA Kit, eif4e1 ELISA Kit, eif4el1 ELISA Kit, EIF-4E ELISA Kit, zgc:101581 ELISA Kit, eukaryotic translation initiation factor 4E ELISA Kit, eukaryotic translation initiation factor 4ea ELISA Kit, Eukaryotic initiation factor 4E ELISA Kit, eucaryotic initiation factor 6 ELISA Kit, eukaryotic translation initiation factor 4E L homeolog ELISA Kit, eukaryotic translation initiation factor 4E family member 1B S homeolog ELISA Kit, translation initiation factor eIF4E ELISA Kit, eukaryotic translation initiation factor 4eb ELISA Kit, EIF4E ELISA Kit, eif4ea ELISA Kit, Eif4e ELISA Kit, eIF-4E ELISA Kit, eif6 ELISA Kit, eif4e ELISA Kit, eif4e.L ELISA Kit, eif4e1b.S ELISA Kit, LOC100533326 ELISA Kit, eif4eb ELISA Kit
- 基因ID
- 1977
- UniProt
- P06730
- 途径
- BCR Signaling
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