PRKAA1 ELISA 试剂盒
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- 抗原 See all PRKAA1 ELISA试剂盒
- PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))
- 抗原表位
- pSer487, total
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适用
- 人, 小鼠, 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human, Mouse and Rat Phospho-AMPKa1 (Ser487) and Total AMPKa1 ELISA Kit. This assay semi-quantitatively measures AMPKa1 phosphorylated at Serine-487 as well as total AMPKa1 in cell lysate samples.
- 样品类型
- Cell Culture Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- This ELISA kit recognizes Human, Mouse and Rat AMPKa1 phosphorylated at site Serine-487 as well as total AMPKa1.
- 产品特性
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) ELISA Kit
PRKAA1 适用: 人, 小鼠, 大鼠 pSer487 Colorimetric Sandwich ELISA Cell Culture Lysate
Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) ELISA KitPRKAA1 适用: 人 Colorimetric Competition ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) ELISA KitPRKAA1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) ELISA KitPRKAA1 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) ELISA KitPRKAA1 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) ELISA KitPRKAA1 适用: 小鼠 Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum
Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) ELISA KitPRKAA1 适用: 大鼠 Colorimetric Sandwich ELISA 3.125-200 ng/mL Plasma, Serum
Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) ELISA KitPRKAA1 适用: 人 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Plasma, Serum
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all PRKAA1 ELISA试剂盒
- PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))
- 别名
- AMPKa1 (PRKAA1 产品)
- 别名
- AMPK ELISA Kit, AMPKa1 ELISA Kit, cb116 ELISA Kit, im:7154392 ELISA Kit, sb:cb130 ELISA Kit, wu:fa94c10 ELISA Kit, AMPK1 ELISA Kit, AMPKalpha1 ELISA Kit, AI194361 ELISA Kit, AI450832 ELISA Kit, AL024255 ELISA Kit, C130083N04Rik ELISA Kit, ampk ELISA Kit, ampka1 ELISA Kit, PRKAA1 ELISA Kit, protein kinase AMP-activated catalytic subunit alpha 1 ELISA Kit, protein kinase, AMP-activated, alpha 1 catalytic subunit ELISA Kit, protein kinase, AMP-activated, alpha 1 catalytic subunit L homeolog ELISA Kit, PRKAA1 ELISA Kit, prkaa1 ELISA Kit, Prkaa1 ELISA Kit, prkaa1.L ELISA Kit
- 基因ID
- 5562
- UniProt
- Q13131, Q5EG47, P54645
- 途径
- AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect
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