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FOXO3 ELISA 试剂盒

FOXO3 适用: 人, 小鼠 pSer263 Colorimetric Sandwich ELISA Cell Culture Lysate
产品编号 ABIN5526709
发货至: 中国
  • 抗原 See all FOXO3 ELISA试剂盒
    FOXO3 (Forkhead Box O3 (FOXO3))
    抗原表位
    pSer263
    适用
    • 11
    • 9
    • 4
    • 1
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    人, 小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human and Mouse Phospho-FOXO3 (Ser253) ELISA Kit. This assay semi-quantitatively measures FOXO3 phosphorylated at Serine-263 in cell lysate samples.
    样品类型
    Cell Culture Lysate
    Analytical Method
    Semi-Quantitative
    特异性
    This ELISA kit recognizes Human and Mouse FOXO3 phosphorylated at site Serine-263.
    产品特性
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    试剂未包括
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Featured
    Discover our best selling FOXO3 ELISA Kit
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    有效期
    6 months
  • 抗原 See all FOXO3 ELISA试剂盒
    FOXO3 (Forkhead Box O3 (FOXO3))
    别名
    FOXO3 (FOXO3 产品)
    别名
    AF6q21 ELISA Kit, FKHRL1 ELISA Kit, FKHRL1P2 ELISA Kit, FOXO2 ELISA Kit, FOXO3A ELISA Kit, zgc:92176 ELISA Kit, wu:fb50g02 ELISA Kit, wu:fi33f07 ELISA Kit, Fkhrl1 ELISA Kit, Foxo3a ELISA Kit, FoxO3A ELISA Kit, 1110048B16Rik ELISA Kit, 2010203A17Rik ELISA Kit, C76856 ELISA Kit, Fkhr2 ELISA Kit, FOXO-3 ELISA Kit, FOXO3 ELISA Kit, foxo3a ELISA Kit, xfoxo3 ELISA Kit, foxO3 ELISA Kit, foxO ELISA Kit, fkhr ELISA Kit, foxo5 ELISA Kit, wu:fj65a12 ELISA Kit, zFkhr ELISA Kit, forkhead box O3 ELISA Kit, forkhead box O3A ELISA Kit, forkhead box O3 L homeolog ELISA Kit, forkhead box O3b ELISA Kit, FOXO3 ELISA Kit, foxo3a ELISA Kit, Foxo3 ELISA Kit, foxo3.L ELISA Kit, foxo3 ELISA Kit, foxO3 ELISA Kit, foxo3b ELISA Kit
    基因ID
    2309
    UniProt
    O43524, Q9WVH4
    途径
    Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis
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