SMAD5 ELISA 试剂盒
SMAD5
适用: 人
pSer463
Colorimetric
Cell ELISA
Adherent Cell Culture
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- 抗原 See all SMAD5 ELISA试剂盒
- SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))
- 抗原表位
- pSer463, pSer465, total
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Cell ELISA
- 应用范围
- ELISA
- 原理
- Cell-Based ELISA Kit. This assay semi-quantitatively measures SMAD5 phosphorylated at Serine-463/465 as well as total SMAD5 in adherent cell lines.
- 样品类型
- Adherent Cell Culture
- Analytical Method
- Semi-Quantitative
- 特异性
- This ELISA kit recognizes Human SMAD5 phosphorylated at site Serine-463/465 as well as total SMAD5.
- 产品特性
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- Rapidly measure phosphorylated protein in adherent cell lines
- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- No sample lysis is needed
- Compatible with a standard ELISA plate reader
- 组件
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- Uncoated 96-well Strip Microplate
- Wash Buffers
- Fixing Solution
- Quenching Buffer
- Blocking Buffer
- Anti-phospho antibody
- Anti-pan antibody
- HRP-Conjugated Secondary Antibody
- TMB One-Step Substrate
- Stop Solution
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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SMAD, Mothers Against DPP Homolog 5 (SMAD5) ELISA Kit
SMAD5 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all SMAD5 ELISA试剂盒
- SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))
- 别名
- SMAD5 (SMAD5 产品)
- 别名
- DWFC ELISA Kit, JV5-1 ELISA Kit, MADH5 ELISA Kit, Madh5 ELISA Kit, SBN ELISA Kit, fb67b04 ELISA Kit, mad ELISA Kit, madh5 ELISA Kit, sb:cb400 ELISA Kit, smd5 ELISA Kit, wu:fb67b04 ELISA Kit, SMAD5 ELISA Kit, DKFZp468M0619 ELISA Kit, 1110051M15Rik ELISA Kit, AI451355 ELISA Kit, Dwf-C ELISA Kit, MusMLP ELISA Kit, SMAD family member 5 ELISA Kit, SMAD5 ELISA Kit, Smad5 ELISA Kit, smad5 ELISA Kit
- 基因ID
- 4090
- UniProt
- Q99717
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