HDAC2 ELISA 试剂盒
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- 抗原 See all HDAC2 ELISA试剂盒
- HDAC2 (Histone Deacetylase 2 (HDAC2))
- 抗原表位
- pSer394, total
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适用
- 人, 小鼠, 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human, Mouse and Rat Phospho-HDAC2 (Ser394) and Total HDAC2 ELISA Kit. This assay semi-quantitatively measures HDAC2 phosphorylated at Serine-394 as well as total HDAC2 in cell lysate samples.
- 样品类型
- Cell Culture Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- This ELISA kit recognizes Human, Mouse and Rat HDAC2 phosphorylated at site Serine-394 as well as total HDAC2.
- 产品特性
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- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Top Product
- Discover our top product HDAC2 ELISA Kit
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Histone Deacetylase 2 (HDAC2) ELISA Kit
HDAC2 适用: 人, 小鼠, 大鼠 pSer394 Colorimetric Sandwich ELISA Cell Culture Lysate
Histone Deacetylase 2 (HDAC2) ELISA KitHDAC2 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Histone Deacetylase 2 (HDAC2) ELISA KitHDAC2 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Histone Deacetylase 2 (HDAC2) ELISA KitHDAC2 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Histone Deacetylase 2 (HDAC2) ELISA KitHDAC2 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Histone Deacetylase 2 (HDAC2) ELISA KitHDAC2 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Histone Deacetylase 2 (HDAC2) ELISA KitHDAC2 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Histone Deacetylase 2 (HDAC2) ELISA KitHDAC2 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all HDAC2 ELISA试剂盒
- HDAC2 (Histone Deacetylase 2 (HDAC2))
- 别名
- HDAC2 (HDAC2 产品)
- 别名
- HD2 ELISA Kit, RPD3 ELISA Kit, YAF1 ELISA Kit, D10Wsu179e ELISA Kit, Yy1bp ELISA Kit, mRPD3 ELISA Kit, HISTONE DEACETYLASE ELISA Kit, T1N24.9 ELISA Kit, T1N24_9 ELISA Kit, histone deacetylase 2 ELISA Kit, histone deacetylase 2 ELISA Kit, histone deacetylase 2 S homeolog ELISA Kit, HDAC2 ELISA Kit, Hdac2 ELISA Kit, hdac2.S ELISA Kit, HDA2 ELISA Kit
- 基因ID
- 3066
- UniProt
- Q92769, P70288, F7ENH8
- 途径
- Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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