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CREB1 ELISA 试剂盒

CREB1 适用: 人 phosphorylated Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
产品编号 ABIN5526692
发货至: 中国
  • 抗原 See all CREB1 ELISA试剂盒
    CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))
    抗原表位
    phosphorylated
    适用
    • 10
    • 8
    • 7
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    DNA-Binding ELISA
    应用范围
    ELISA
    原理
    Human pCREB Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical CREB binding sequences phosphorylated at site serine residue 133 to semi-quantitatively detect active pCREB in lysates or nuclear extracts. Only available in North America.
    样品类型
    Cell Lysate, Nuclear Extract
    Analytical Method
    Semi-Quantitative
    特异性
    The olionucleotide/antibody pair provided in this kit recognizes human phosphorylated CREB in whole lysates and nuclear extracts.
    产品特性
    • Specific transcription factor-DNA binding assay
    • Perfect alternative to EMSA
    • Easy to perform in an ELISA format
    • Non-radioactive assay
    • High throughput (96 well plate format)
    • Assay can be completed within 5 hours
    组件
    • 96-well Strip Microplate pre-coated with DNA probes
    • DNA Binding Buffer
    • Positive Control Sample
    • Specific Competitor DNA probe
    • Non-specific Competitor DNA probe
    • Assay Reagent
    • DTT
    • Wash Buffer
    • Primary Antibody
    • HRP-conjugated Secondary Antibody
    • TMB One-Step Substrate Reagent
    • Stop Solution
    试剂未包括
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions Absorbent paper
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • 应用备注
    Optimal working dilution should be determined by the investigator.
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared HRP-secondary antibody to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.
    有效期
    6 months
  • 抗原 See all CREB1 ELISA试剂盒
    CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))
    别名
    CREB (CREB1 产品)
    别名
    creb ELISA Kit, CREB1 ELISA Kit, Creb1 ELISA Kit, CREB ELISA Kit, 2310001E10Rik ELISA Kit, 3526402H21Rik ELISA Kit, AV083133 ELISA Kit, Creb ELISA Kit, Creb-1 ELISA Kit, creb1 ELISA Kit, zgc:55598 ELISA Kit, cAMP responsive element binding protein 1 ELISA Kit, cAMP responsive element binding protein ELISA Kit, cAMP responsive element binding protein 1 S homeolog ELISA Kit, cAMP responsive element binding protein 1a ELISA Kit, CREB1 ELISA Kit, creb1 ELISA Kit, CREB ELISA Kit, Creb1 ELISA Kit, creb1.S ELISA Kit, creb1a ELISA Kit
    基因ID
    1385
    UniProt
    P16220
    途径
    TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation
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