NOS2 ELISA 试剂盒
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- 抗原 See all NOS2 ELISA试剂盒
- NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))
- 抗原表位
- phosphorylated
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适用
- 人, 小鼠, 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human, Mouse and Rat Phosphotyrosine iNOS ELISA Kit. This assay semi-quantitatively measures tyrosine-phosphorylated iNOS in cell lysate samples.
- 样品类型
- Cell Culture Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- This ELISA kit recognizes human iNOS phosphotyrosine.
- 产品特性
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Biotinylated Anti-Phosphotyrosine Antibody
- Stop Solution
- Assay Diluent(s)
- Positive Control Sample
- Lysis Buffer
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Biotinylated Anti-Phosphotyrosine Antibody
- Stop Solution
- Assay Diluent(s)
- Positive Control Sample
- Lysis Buffer
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Featured
- Discover our best selling NOS2 ELISA Kit
- Top Product
- Discover our top product NOS2 ELISA Kit
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Nitric Oxide Synthase 2, Inducible (NOS2) ELISA Kit
NOS2 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 适用: 人 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 适用: 兔 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 适用: 大鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitWide Range NOS2 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all NOS2 ELISA试剂盒
- NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))
- 别名
- iNOS (NOS2 产品)
- 别名
- HEP-NOS ELISA Kit, INOS ELISA Kit, NOS ELISA Kit, NOS2A ELISA Kit, NOS-II ELISA Kit, Nos-2 ELISA Kit, Nos2a ELISA Kit, i-NOS ELISA Kit, iNOS ELISA Kit, iNos ELISA Kit, NOS2a ELISA Kit, NOS2 ELISA Kit, BmNOS2 ELISA Kit, Nsl ELISA Kit, nos2 ELISA Kit, nitric oxide synthase 2 ELISA Kit, nitric oxide synthase 2, inducible ELISA Kit, inducible nitric oxide synthase ELISA Kit, inducible nitric-oxide synthase ELISA Kit, nanos C2HC-type zinc finger 2 ELISA Kit, NOS2 ELISA Kit, Nos2 ELISA Kit, nos2 ELISA Kit, LOC396821 ELISA Kit, NANOS2 ELISA Kit
- 基因ID
- 4843
- UniProt
- P35228, P29477, Q06518
- 途径
- Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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