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ULK1 ELISA 试剂盒

ULK1 适用: 人, 小鼠 pSer556 Colorimetric Sandwich ELISA Cell Culture Lysate
产品编号 ABIN5526680
发货至: 中国
  • 抗原 See all ULK1 ELISA试剂盒
    ULK1 (Unc-51 Like Kinase 1 (ULK1))
    抗原表位
    pSer556
    适用
    人, 小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human and Mouse Phospho-ULK1 (S556) ELISA Kit. This assay semi-quantitatively measures ULK1 phosphorylated at Serine-556 in cell lysate samples.
    样品类型
    Cell Culture Lysate
    Analytical Method
    Semi-Quantitative
    特异性
    This ELISA kit recognizes Human and Mouse ULK1 phosphorylated at site Serine-556.
    产品特性
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    试剂未包括
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Featured
    Discover our best selling ULK1 ELISA Kit
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    有效期
    6 months
  • 抗原 See all ULK1 ELISA试剂盒
    ULK1 (Unc-51 Like Kinase 1 (ULK1))
    别名
    ULK1 (ULK1 产品)
    别名
    ATG1 ELISA Kit, ATG1A ELISA Kit, UNC51 ELISA Kit, Unc51.1 ELISA Kit, hATG1 ELISA Kit, si:dkey-11o18.7 ELISA Kit, ulk1 ELISA Kit, ULK1 ELISA Kit, AU041434 ELISA Kit, mKIAA0722 ELISA Kit, zgc:195008 ELISA Kit, unc-51 like autophagy activating kinase 1 ELISA Kit, unc-51 like kinase 1 ELISA Kit, unc-51 like autophagy activating kinase 1a ELISA Kit, ULK1 ELISA Kit, Ulk1 ELISA Kit, ulk1b ELISA Kit, ulk1a ELISA Kit
    基因ID
    8408
    UniProt
    O75385, O70405
    途径
    Regulation of Cell Size, Autophagy
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