Neopterin ELISA 试剂盒
-
- 抗原 See all Neopterin (NP) products
- Neopterin (NP)
-
适用
- 化学剂
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 应用范围
- ELISA
- 样品类型
- Serum
- 灵敏度
- 0.2 ng/mL
- 产品特性
- Neopterin, Human, ELISA Kit
- 组件
-
- Micrototer Plate, 1x96 wells
- Standard 0nM, 1x1.5ml
- Standard 1.35nM, 1x1.5ml
- Standard 4nM, 1x1.5ml
- Standard 12nM, 1x1.5ml
- Standard 37nM, 1x1.5ml
- Standard 111nM, 1x1.5ml
- Assay Buffer, 1x21ml
- Control 1, .3nM, 1x1.5ml
- Control 2, 15.nM, 1x1.5ml
- Neopterin (HRP Conjugate, 1x13ml
- Neopterin Antibody, 1x5ml
- Wash Buffer, 20X, 1x50ml
- TMB Substrate Solution, 1x19ml
- Stop Solution, 1N O4, 1x19ml
-
Neopterin (NP) ELISA Kit
NP 适用: 人 Colorimetric Competition ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Neopterin (NP) ELISA KitNP 适用: Cow Colorimetric Competition ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Neopterin (NP) ELISA KitNP 适用: 绵羊 Colorimetric Competition ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
-
- 实验流程
-
Principle:
- Neopterin ELISA kit is based on competitive binding of human Neopterin from serum samples and enzyme-labeled Neopterin to Neopterin specific antibodies immobilized on microtiter plates. After a washing step, chromogenic substrate is added and color developed. The enzymatic reaction (blue color is inversely proportional to the amount of Neopterin present in the sample. The reaction is terminated by adding stopping solution (converts blue to yellow. Absorbance is then measured on an ELISA reader at 450nm and the concentration of Neopterin in samples and control is read off the standard curve.
- 实验精密度
-
Precision CV: < 10% for Neopterin range 2-50nmol/L
- Inter-assay Precision CV: < 10% for Neopterin range 2-50nmol/L
- 限制
- 仅限研究用
-
- 储存条件
- 4 °C
- 储存方法
- 4°C
-
- 抗原 See all Neopterin (NP) products
- Neopterin (NP)
- 别名
- Neopterin (NP 产品)
- 物质类
- Chemical
- 背景
- Neopterin [D-erythro-neopterin] belongs to the group of pteridines. Neopterin as well as other pteridines are derived in vivo from guanosine-triphophate (GTP). Neopterin represents a precursor molecule of biopterin that is an essential cofactor in neurotransmitter synthesis, and it is also involved in variety of oxidation-reduction reactions in the body. The enzyme GTP-cyclohydrolase-I catalyzes this reaction in monocytes and macrophages. Neopterin is exclusively released by activated macrophages, and can be determined in serum, plasma and other body fluids. Neopterin biosynthesis is closely associated with activation of the cellular immune system. Higher levels of neopterin were found in patients with viral infections. Antigenic stimulation of human peripheral blood mononuclear cells leads to neopterin release into cell culture medium. Gamma Interferon treatment of macrophages in vitro also increases neopterin levels and generates highly reactive oxygen species (ROS). Neopterin is capable of enhancing ONOO- - as well as Cu(II)-mediated LDL oxidation, whereas 7,8-dihydroneopterin mainly protects LDL from oxidation.
-
