HAMA ELISA 试剂盒
HAMA
适用: 人, 小鼠
Colorimetric
Sandwich ELISA
Serum
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- 抗原 See all HAMA products
- HAMA (Human Anti-Mouse Antibody (HAMA))
- 适用
- 人, 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 样品类型
- Serum
- 灵敏度
- > 3 ng/mL
- 产品特性
- HAMA (Human anti-Mouse Antibody) ELISA Kit
- 组件
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- HAMA Microtiter Plate, Pre-coated with mouse IgG. 1x96 wells
- HRP Conjugate, Mouse IgG labeled with Horseradish Peroxidase (HRP). 1x12ml
- * HAMA Standard, Rabbit anti-Mouse IgG antibody, supplied as a lyophilized powder from a buffered protein base with preservative. 2x800ng
- Calibrator Diluent, Animal serum with buffer and preservative. 1x25ml
- Wash Buffer, 20X, 20-fold concentrated solution of buffered surfactant. 1x60ml
- Substrate A, Buffered solution with 2. 1x10ml
- Substrate B, Buffered solution with TMB. 1x10ml
- Stop Solution, 2N Sulphuric Acid (O4). 1x15ml
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Human Anti-Mouse Antibody (HAMA) ELISA Kit
HAMA 适用: 人 Colorimetric Sandwich ELISA 2.0-1500 ng/mL Plasma, Serum
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- 板类型
- Pre-coated
- 实验流程
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Principle:
- This assay applies an enzyme-linked immunosorbent assay (ELISA) technique. A purified mouse IgG has been pre-coated onto the wells of microplate. Standard (rabbit anti-mouse IgG) and specimens are added into the appropriate wells simultaneously with a HRP-labelled mouse IgG, and incubated. The Human Anti-Mouse Antibody (HAMA), if present, will bind with the mouse IgG which coated on the wells and HRP- labelled mouse IgG as well, forming a "Sandwich". Following a wash to remove any unbound substance, a substrate solution is added. The developed colour is directly proportional to the anti-mouse antibody concentration in the specimen and Standard samples.
- 限制
- 仅限研究用
-
- 储存条件
- 4 °C,-20 °C
- 储存方法
- 4°C/-20°C
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- 抗原 See all HAMA products
- HAMA (Human Anti-Mouse Antibody (HAMA))
- 别名
- HAMA (Human anti-Mouse Antibody) (HAMA 产品)
- 物质类
- Antibody
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