beta Amyloid ELISA 试剂盒
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- 抗原 See all beta Amyloid (Abeta) ELISA试剂盒
- beta Amyloid (Abeta) (Amyloid beta (Abeta))
- 抗原表位
- AA 1-40
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- The Human beta Amyloid 1-40 (Hu A beta40) ELISA kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for the NH2-terminus of Hu b-amyloid has been coated onto the wells of the microtiter strips provided. During the first incubation, standards of known Hu b-amyloid 40 content, controls, and unknown samples are pipetted into the wells and co-incubated with a rabbit antibody specific for the COOH-terminus of the 1-40 b-amyloid sequence. This COOH- terminal sequence is created upon cleavage of the analyzed precursor. Bound rabbit antibody is detected by the use of a horseradish peroxidase-labeled anti-rabbit antibody. After washing, horseradish peroxidase-labeled anti-rabbit antibody (enzyme) is added. After a second incubation and washing to remove all the unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Hu A beta40 present in the original specimen.
- 样品类型
- Cerebrospinal Fluid, Tissue Homogenate, Tissue Culture Medium
- Analytical Method
- Quantitative
- 交叉反应 (详细)
- Calculated cross reactivity: Hu
- 灵敏度
- > 6 pg/mL
- 产品特性
- β-Amyloid, aa1-40, Human, ELISA Kit
- 组件
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- * beta Amyloid Standard, 1x1vial
- Standard Diluent Buffer, 1x60ml
- Microtiter Plate, 1x96 wells
- beta Amyloid , Pab 1x6ml
- IgG (HRP) (100X), 1x125ul
- HRP Diluent, 1x25ml
- Wash Buffer (25X), 1x100ml
- TMB, 1x25ml
- Stop Solution, 1x25ml
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- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-80 °C
- 储存方法
- 4°C/-70°C
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- 抗原 See all beta Amyloid (Abeta) ELISA试剂盒
- beta Amyloid (Abeta) (Amyloid beta (Abeta))
- 别名
- beta-Amyloid (Abeta 产品)
- 别名
- AAA ELISA Kit, ABETA ELISA Kit, ABPP ELISA Kit, AD1 ELISA Kit, APPI ELISA Kit, CTFgamma ELISA Kit, CVAP ELISA Kit, PN-II ELISA Kit, PN2 ELISA Kit, aaa ELISA Kit, abeta ELISA Kit, abpp ELISA Kit, ad1 ELISA Kit, appi ELISA Kit, ctfgamma ELISA Kit, cvap ELISA Kit, pn2 ELISA Kit, Abeta ELISA Kit, Abpp ELISA Kit, Adap ELISA Kit, Ag ELISA Kit, Cvap ELISA Kit, E030013M08Rik ELISA Kit, betaApp ELISA Kit, amyloid beta precursor protein ELISA Kit, amyloid beta (A4) precursor protein ELISA Kit, APP ELISA Kit, app ELISA Kit, App ELISA Kit
- 途径
- Inflammasome
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