Phenylalanine Hydroxylase ELISA 试剂盒
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- 抗原 See all Phenylalanine Hydroxylase ELISA试剂盒
- Phenylalanine Hydroxylase
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适用
- 化学剂
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- PAH (Phenylalanine Hydroxylase) ELISA Kit is a quantitative sandwich assay for the detection of PAH in human serum, plasma, tissue homogenates and other biological fluids
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 灵敏度
- 46.875 pg/mL
- 产品特性
- PAH (Phenylalanine Hydroxylase) ELISA Kit (Human)
- 组件
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- *357883A: Microtiter Strips, 1x96 wells (8x12 wells).
- *357883B: Standard, 2x1 vial
- 357883C: Sample/Standard Dilution Buffer, 1x20ml
- 357883D: Antibody (Biotin) (Concentrated), 1x120ul
- 357883E: Antibody Dilution Buffer, 1x10ml
- 357883F: Streptavidin (HRP) (SABC), 1x120ul
- 357883G: SABC Dilution Buffer, 1x10ml
- 357883H: TMB Substrate, 1x10ml
- 357883J: Stop Solution, 1x10ml
- 357883K: Wash Buffer, 25X, 1x30ml
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- Discover our best selling Phenylalanine Hydroxylase ELISA Kit
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Phenylalanine Hydroxylase ELISA Kit
适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Phenylalanine Hydroxylase ELISA Kit适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Phenylalanine Hydroxylase ELISA Kit适用: 人 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Phenylalanine Hydroxylase ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 23.5 pg/mL - 1500 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Phenylalanine Hydroxylase ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Phenylalanine Hydroxylase ELISA Kit适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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- 板类型
- Pre-coated
- 实验流程
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Principle:
- This ELISA kit employs the sandwich enzyme-linked immunoassay technique, utilizing a microtiter plate pre-coated with an antibody specific to PAH. Standards and samples are added to the appropriate wells, then incubated. Aspirate. Do not wash the plate! The Antibody (Biotin) is added to all the wells. The plate is incubated and then washed. Streptavidin (HRP) is added to each microplate well and incubated then washed. Then the TMB substrate solution is added and incubated. After the TMB substrate solution is added, only those wells that contain PAH, the antibody (Biotin) and Streptavidin (HRP) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PAH in the sample is then determined by comparing the O.D. of the sample to the standard curve.
- 实验精密度
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Precision:
- Intra-Assay CV: <8%
- Inter-Assay CV: <10%
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- 4°C/-20°C
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- 抗原 See all Phenylalanine Hydroxylase ELISA试剂盒
- Phenylalanine Hydroxylase
- 别名
- PAH (Phenylalanine Hydroxylase) (Phenylalanine Hydroxylase 产品)
- 别名
- cb877 ELISA Kit, wu:fb02f10 ELISA Kit, zgc:65981 ELISA Kit, GB18494 ELISA Kit, PAH ELISA Kit, pku ELISA Kit, pku1 ELISA Kit, DDBDRAFT_0206188 ELISA Kit, DDBDRAFT_0231664 ELISA Kit, DDB_0206188 ELISA Kit, DDB_0231664 ELISA Kit, AW106920 ELISA Kit, PH ELISA Kit, PKU ELISA Kit, PKU1 ELISA Kit, phenylalanine-4-hydroxylase ELISA Kit, phenylalanine hydroxylase ELISA Kit, protein henna ELISA Kit, phenylalanine 4-monooxygenase ELISA Kit, pah ELISA Kit, LOC408622 ELISA Kit, PAH ELISA Kit, CpipJ_CPIJ002149 ELISA Kit, Pah ELISA Kit
- 物质类
- Chemical
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