FLOW IntraCellular Staining Kit
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- 检测方法
- Fluorometric
- 应用范围
- Flow Cytometry (FACS)
- 原理
- Flowcytometry staining kits
- 组件
- Kit Components: Fixation Buffer (1X) - 60 ml, Permeabilization Buffer (10X)- 2 X 60 ml, Staining Buffer - (1X)- 60 ml
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Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
TNF alpha 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Lipopolysaccharides (LPS) ELISA Kit适用: Various Species Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 人 AA 77-233 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 6 (IL6) ELISA KitIL6 适用: 人 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 适用: 人 Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 实验流程
- The Intracellular FLOW Staining Kit allows detection antibodies to access the intracellular protein in Flowcytometry analysis. This kit is specially formulated to reduce non-specific staining of fluorochrome-labelled antibodies and to increase fluorescence signaling. First, live cells can be fixed with the Fixation buffer, which cross-links the protein. Second, the 1X Permeabilization Buffer creates holes in the membrane, allowing the antibodies enter into the cells effectively. Washing steps and antibody dilutions are done using 1X Permeabilization Buffer. The final cell resuspension prior to analysis is done using The Staining Buffer. Permeabilization Buffer is supplied as a 10X solution. Prior to use, this should be diluted 10 fold in distilled water. (1 mL of Permeabilization Buffer with 9 mL of distilled water). 10X Permeabilization buffer has natural tendency to precipitate, however, its function is not affected. 1X solution can be filtered.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C
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