PD-L1 ELISA 试剂盒
-
- 抗原 See all PD-L1 ELISA试剂盒
- PD-L1 (CD274 (PD-L1))
-
适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156-10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 原理
- This immunoassay kit allows for the in vitro quantitative determination of Mouse PDL-1 concentrations in serum, plasma and other biological fluids.
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 灵敏度
- < 0.094 ng/mL
- 组件
- plate, standard, diluent, antibodies
- 试剂未包括
- pipettes, tubes, reader, incubator
- Featured
- Discover our best selling PD-L1 ELISA Kit
- Top Product
- Discover our top product PD-L1 ELISA Kit
-
-
- 样本量
- 100 μL
- 实验时间
- 3 - 4 h
- 板类型
- Pre-coated
- 实验流程
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Mouse PDL-1 antibody was pre-coated into 96-well plates. Biotin conjugated anti-MousePDL-1detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse PDL-1 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Mouse PDL-1 can be calculated using the standard curve.
- 限制
- 仅限研究用
-
- 注意事项
- protect your eyes
- 储存条件
- 4 °C
-
- 抗原 See all PD-L1 ELISA试剂盒
- PD-L1 (CD274 (PD-L1))
- 别名
- PD-L1 (PD-L1 产品)
- 途径
- Cancer Immune Checkpoints
-