PD-1 ELISA 试剂盒
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- 抗原 See all PD-1 (PDCD1) ELISA试剂盒
- PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156-10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 原理
- This immunoassay kit allows for the in vitro quantitative determination of Human PDL1 concentrations in serum, plasma and other biological fluids. This kit is for Research use only. Not for use in diagnostic/therapeutics procedures.
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 灵敏度
- < 0.094 ng/mL
- 组件
- plate, standard, diluent, antibodies
- 试剂未包括
- pipettes, tubes, reader, incubator
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- Discover our top product PDCD1 ELISA Kit
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- 样本量
- 100 μL
- 实验时间
- 3 - 4 h
- 板类型
- Pre-coated
- 实验流程
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human PD-1 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human PD-1 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PD-1 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human PD-1 can be calculated using the standard curve.
- 限制
- 仅限研究用
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- 注意事项
- protect your eyes
- 储存条件
- 4 °C
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- 抗原 See all PD-1 (PDCD1) ELISA试剂盒
- PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
- 别名
- PD-1 (Programmed Cell Death Protein1) (PDCD1 产品)
- 别名
- CD279 ELISA Kit, PD-1 ELISA Kit, PD1 ELISA Kit, SLEB2 ELISA Kit, hPD-1 ELISA Kit, hPD-l ELISA Kit, Ly101 ELISA Kit, Pdc1 ELISA Kit, programmed cell death 1 ELISA Kit, PDCD1 ELISA Kit, Pdcd1 ELISA Kit
- 途径
- Cancer Immune Checkpoints
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