MIF ELISA 试剂盒
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- 抗原 See all MIF ELISA试剂盒
- MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.781-50 ng/mL
- 最低检测浓度
- 0.781 ng/mL
- 应用范围
- ELISA
- 原理
- This immunoassay kit allows for the in vitro quantitative determination of Human MIF concentrations in serum, plasma and other biological fluids.
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 灵敏度
- < 0.469 ng/mL
- 组件
- plate, standard, diluent, antibodies
- 试剂未包括
- pipettes, tubes, reader, incubator
- Top Product
- Discover our top product MIF ELISA Kit
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- 样本量
- 100 μL
- 实验时间
- 3 - 4 h
- 板类型
- Pre-coated
- 实验流程
- This assay employs an antibody specific for Anti-human MIF coated on a 96-well plate. Standards and samples are pipetted into the wells and MIF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- 限制
- 仅限研究用
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- 注意事项
- protect your eyes
- 储存条件
- 4 °C
-
- 抗原 See all MIF ELISA试剂盒
- MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))
- 别名
- MIF (Macrophage migration inhibitory factor) (MIF 产品)
- 途径
- Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
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