Thymic Stromal Lymphopoietin ELISA 试剂盒
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- 抗原 See all Thymic Stromal Lymphopoietin (TSLP) ELISA试剂盒
- Thymic Stromal Lymphopoietin (TSLP)
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.02-1.0 ng/mL
- 最低检测浓度
- 0.02 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
- Natural and recombinant Mouse TSLP Ligand
- 灵敏度
- 7 pg/mL
- 试剂未包括
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- Microplate reader.
- Pipettes and pipette tips.
- EP tube Deionized or distilled water.
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- 应用备注
- Detection Wavelength: 450 nm
- 样本量
- 20 μL
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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- 抗原 See all Thymic Stromal Lymphopoietin (TSLP) ELISA试剂盒
- Thymic Stromal Lymphopoietin (TSLP)
- 别名
- TSLP (TSLP 产品)
- 别名
- TSLP ELISA Kit, thymic stromal lymphopoietin ELISA Kit, TSLP ELISA Kit, Tslp ELISA Kit
- 背景
- Thymic Stromal Lymphopoietin (TSLP) is a member of the B cell-stimulating factor family. Originally identified from the conditioned medium of a mouse thymic stromal cell line that promoted B-cell development, TSLP is expressed in thymus, spleen, kidney, lung, and bone marrow. Mouse TSLP cDNA encodes a 140 amino acid (aa) residue precursor protein with a 19 aa signal sequence. TSLP activity overlaps with that of IL-7, playing a key role in the development of B cells, as well as stimulating the growth of thymocytes and T cells. TSLP stimulates monocytes to produce T cell-attracting chemokines such as TARC (CCL17) and MDC (CCL22). TSLP also activates dendritic cell-primed CD4 T cells to produce Th2 cytokines, including IL-4, IL-5, IL-13, and TNFα. This hematopoietic cytokine binds to a heterodimeric receptor complex consisting of the IL-7R alpha chain (IL-7Rα) and the TSLP-specific chain (TSLPR). Binding induces activation of STAT3 and STAT5 via phosphorylation. Mouse TSLP shares approximately 43 % amino acid sequence identity with human TSLP.
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