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CD40 Ligand ELISA 试剂盒

CD40LG 适用: 人 Colorimetric Sandwich ELISA 156.25-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
产品编号 ABIN4987074
发货至: 中国
  • 抗原 See all CD40 Ligand (CD40LG) ELISA试剂盒
    CD40 Ligand (CD40LG)
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    156.25-10000 pg/mL
    最低检测浓度
    156.25 pg/mL
    应用范围
    ELISA
    样品类型
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)
    Analytical Method
    Quantitative
    特异性
    Natural and recombinant Human sCD40L Ligand
    灵敏度
    40 pg/mL
    试剂未包括
    • Microplate reader.
    • Pipettes and pipette tips.
    • EP tube Deionized or distilled water.
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  • 应用备注
    Detection Wavelength: 450 nm
    样本量
    20 μL
    实验时间
    3 h
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    4 °C
  • 抗原 See all CD40 Ligand (CD40LG) ELISA试剂盒
    CD40 Ligand (CD40LG)
    别名
    sCD40L (CD40LG 产品)
    背景
    CD40 Ligand (CD40L), also known as CD154, gp39, TNFSF5, TRAP (TNF-Related Activation Protein) or TBAM (T-cell B-cell Activating Molecule), is a multifunctional ligand in the TNF superfamily (1-4). Interaction between CD40 and CD40L is critical to the control of thymus-dependent humoral immunity and cell-mediated immune responses (5-10). The major component of the contact-dependent signal leading to B cell activation is CD40L. CD40L stimulates B cell secretion of immunoglobulin isotypes in the presence of cytokines.CD40L is a 39 kDa, 261 amino acid (aa) glycoprotein that can form homotrimers typical of other TNFSF members (1-4, 11, 12). Proteolytic cleavage can also produce 15-18 kDa soluble forms of CD40L (13, 14). Activated T cells and platelets express both a membrane-associated and a soluble form of CD40L (sCD40L) (13, 15, 16). Platelet activation during plasma and serum sample preparation can result in artificially elevated sCD40L levels (17-20). Conversely, serum samples stored above 2-8
    途径
    NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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