Interleukin 17a ELISA 试剂盒
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- 抗原 See all Interleukin 17a (IL17A) ELISA试剂盒
- Interleukin 17a (IL17A) (Interleukin 17A (IL17A))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 7.8-500 pg/mL
- 最低检测浓度
- 7.8 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
- Natural and recombinant Mouse IL-17A Ligand
- 灵敏度
- 4 pg/mL
- 试剂未包括
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- Microplate reader.
- Pipettes and pipette tips.
- EP tube Deionized or distilled water.
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- Discover our best selling IL17A ELISA Kit
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- Discover our top product IL17A ELISA Kit
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Interleukin 17A (IL17A) ELISA Kit
IL17A 适用: 大鼠 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 人 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Culture Supernatant, Serum, Tissue Homogenate, Urine
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 小鼠 AA 22-158 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 人 AA 20-155 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 小鼠 Colorimetric Sandwich ELISA 6-600 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 大鼠 Colorimetric Sandwich ELISA 3.13-200 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Detection Wavelength: 450 nm
- 样本量
- 20 μL
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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- 抗原 See all Interleukin 17a (IL17A) ELISA试剂盒
- Interleukin 17a (IL17A) (Interleukin 17A (IL17A))
- 别名
- IL-17A (IL17A 产品)
- 别名
- CTLA8 ELISA Kit, IL-17 ELISA Kit, IL-17A ELISA Kit, IL17 ELISA Kit, Ctla-8 ELISA Kit, Ctla8 ELISA Kit, Il17 ELISA Kit, ChIL-17 ELISA Kit, IL-17F ELISA Kit, IL17A ELISA Kit, CTLA-8 ELISA Kit, interleukin 17A ELISA Kit, IL17A ELISA Kit, Il17a ELISA Kit
- 背景
- Mouse Interleukin 17 (IL-17, also known as IL-17A and CTLA-8) is a 21 kDa, variably glycosylated polypeptide that belongs to the IL-17 family of cytokines containing a cysteine-knot fold (1-3). Its sequence was originally isolated from an activated hybridoma created from the fusion of a mouse cytotoxic and rat T cell lymphoma cell line (2-5). It is synthesized as a 158 amino acid (aa) precursor that contains a 25 aa signal sequence and a 15 kDa, 133 aa mature segment (5). In both mouse and human, there is one conserved N-linked glycosylation site that likely contributes 5 kDa to its native molecular weight. IL-17A forms both a 35-38 kDa homodimer,and a 45-48 kDa heterodimer with IL-17F (6, 7). Mature mouse IL-17A is 61 % and 89 % aa identical to human and rat IL-17A, respectively (4, 5, 8). While rodent and human mature sequences show modest aa sequence identity, human IL-17 is active on both mouse and rat cells (5, 9). Cells known to produce IL-17 are the CD4+ Th17 T cells, Paneth cells, GR1+CD11b+ myeloid suppressor cells, CD27-γδ T cells, CD1+NK1.1- iNKT cells and CD3-CD4+ LTi-like cells(3, 5, 6, 10-12).A high affinity receptor for mouse IL-17 has been reported, and appears to be a heteromultimer of IL-17RA and IL-17RC, likely in a 2:1 ratio (1). IL-17RA is a 130 kDa, type I transmembrane glycoprotein that bears no resemblance to members of the cytokine, TNF or immunoglobulin receptor superfamily (2, 10, 13). IL-17RC is also a type I transmembrane protein, approximately 90-95 kDa in size, that shares less than 30 % aa identity with IL-17RA (14, 15). Both receptors are needed for IL-17A and IL-17A/F activity. The two receptors appear to form a functional association following ligand binding to IL-17RA (1, 16).IL-17 is best known for its participation in the recruitment and survival of neutrophils (3, 10, 17,18). Its induction was initially described to be the result of antigen stimulation of dentritic cells, resulting in IL-23 secretion. In a TCR-independent event, IL-23 induces T cell production of IL-17(3). Once secreted, IL-17 in the bone marrow would seem to induce stromal/fibroblast expression of both G-CSF and SCF (membrane form), an effect that increases neutrophil differentiation and activation. IL-17 may complement this by directly blocking neutrophil apoptosis, promoting greater circulating neutrophil numbers (17). In the tissues, IL-17 seems to promote neutrophil extravasation, principally through its effects on macrophages and endothelial cells (EC). On macrophages, IL-17 induces TNF-α, IL-1β and IL-6 production (19).TNF-α and IL-1β then act on local ECs to induce G-CSF secretion, an effect that is potentiated by IL-17 (20). IL-17 further contributes to neutrophil influx by inducing EC CXC chemokine release and NO production, which may increase vascular permeability (3, 9). IL-17 effects are not limited to neutrophils. In synovial joints, IL-17 upregulates RANKL expression on osteoblasts. This provides a stimulus for osteoclast formation and subsequent bone resorption (18).
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