IL-12/IL-23 p40 ELISA 试剂盒
适用: 小鼠
Colorimetric
Sandwich ELISA
7.8-500 pg/mL
Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
1 image
-
- 抗原 See all IL-12/IL-23 p40 products
- IL-12/IL-23 p40
-
适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 7.8-500 pg/mL
- 最低检测浓度
- 7.8 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
- Natural and recombinant Mouse IL-12/IL-23(P40) Ligand
- 灵敏度
- 4 pg/mL
- 试剂未包括
-
- Microplate reader.
- Pipettes and pipette tips.
- EP tube Deionized or distilled water.
-
IL-12/IL-23 p40 ELISA Kit
适用: 人 Colorimetric Sandwich ELISA 156.25-10000 pg/mL Cell Culture Supernatant, Plasma, Serum
IL-12/IL-23 p40 ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 4.69-300 pg/mL Cell Culture Supernatant, Plasma, Serum
-
- 应用备注
- Detection Wavelength: 450 nm
- 样本量
- 20 μL
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 限制
- 仅限研究用
-
- 储存条件
- 4 °C
-
- 抗原 See all IL-12/IL-23 p40 products
- IL-12/IL-23 p40
- 别名
- IL-12/IL-23(P40) (IL-12/IL-23 p40 产品)
- 背景
- IL-12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine produced primarily by antigen-presenting cells (monocytes/macrophages, dendritic cells and B lymphocytes). IL-12 has multiple effects on T lymphocytes and natural killer (NK) cells, including the ability to stimulate cytotoxicity, proliferation, cytokine production and Th1 subset development (1, 2). IL-12 is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a unique 35 kDa (p35) subunit and a common 40 kDa (p40) subunit that is also present in IL-23. Monomers of the p40 and p35 subunits by themselves do not have IL-12 activity, but the homodimer of p40 has been shown to bind the IL-12 receptor and is an IL-12 antagonist (3, 4). In cells expressing both p35 and p40 mRNAs, p40 mRNA is expressed to a higher level and free p40 subunits not associated with p35 subunits are secreted together with heterodimeric IL-12 p70 (5). Most of the free p40 subunits secreted by the various human cell lines examined have been found to exist as monomers (1). In the culture supernates of various activated human monocytes where free p40 is present in vast excess over p70, the levels of p70 measured by bioassays are consistent with those measured using a p70-specific immunoassay, suggesting that p40 monomers are not efficient IL-12 antagonists (1, 6). In the mouse system, p40 homodimers are produced in vivo and function as IL-12 antagonists (7). Polymorphisms exist in the mouse IL-12/IL-23 p40 sequence.
-
You are here:
