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Cytochrome C ELISA 试剂盒

CYCS 适用: 人 Colorimetric Sandwich ELISA 39-2500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
产品编号 ABIN4986817
发货至: 中国
  • 抗原 See all Cytochrome C (CYCS) ELISA试剂盒
    Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    39-2500 pg/mL
    最低检测浓度
    39 pg/mL
    应用范围
    ELISA
    样品类型
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)
    Analytical Method
    Quantitative
    特异性
    Natural and recombinant Human Cytochrome C Ligand
    灵敏度
    10 pg/mL
    试剂未包括
    • Microplate reader.
    • Pipettes and pipette tips.
    • EP tube Deionized or distilled water.
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  • 应用备注
    Detection Wavelength: 450 nm
    样本量
    20 μL
    实验时间
    3 h
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    4 °C
  • 抗原 See all Cytochrome C (CYCS) ELISA试剂盒
    Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))
    别名
    Cytochrome C (CYCS 产品)
    背景
    Human somatic cytochrome c is a 15 kDa, 104 amino acid (aa) polypeptide that participates in both oxidative phosphorylation and apoptosis (1). It exhibits 91 % aa identity with mouse/rat cytochrome c (1-3). The molecule is initially synthesized in the cytoplasm as an extended, 12 kDa apoprotein. This molecule is subsequently transported across the outer mitochondrial membrane into the intermitochondrial space via a receptor-independent process. Here, it associates with an inner mitochondrial membrane enzyme called CCHL (cytochrome c heme lyase) which covalently attaches one heme molecule to the N-terminus, forming functional cytochrome c (4-6). This covalent attachment causes cytochrome c to undergo a conformational change to a globular molecule effectively trapping cytochrome c in the intermitochrondrial space.Within the intermitochondrial space, cytochrome c is actively involved in the oxidative phosphorylation pathway. It transports electrons from the cytochrome c reductase complex to the cytochrome c oxidase complex (7-9). This transports excess electrons along the respiratory pathway and generates ATP for energy-dependent processes. Alternatively, and in response to apoptotic signals, cytochrome c can be released from mitochondria into the cytosol. Here, it activates an apoptotic program via one of many possible caspase-driven cascades (8, 10-12). The events which trigger an apoptotic signal (such as growth factor withdrawal) are not well understood. The result of such a signal, however, has been suggested to be a translocation of cytosolic Bax and/or Bad to the outer mitochondrial membrane where they overcome a Bcl-2-mediated stabilization (13-15). Bax-mediated destabilization may take the form of channel creation or mitochondrial swelling, resulting in cytochrome c release (15-18). Whatever the mechanism, released cytochrome c participates in the formation of a cytosolic complex which is composed of 15 kDa cytochrome c, 130 kDa Apaf-1 (apoptotic protease activating factor-1), dATP, and 46 kDa Apaf-3/caspase-9 (15, 18, 19). Within this complex, Apaf-3/caspase-9 is activated, leading to the downstream activation of caspases-3,-7 and -9, followed by additional caspases that ultimately lead to cellular apoptosis (10, 18, 20,21).
    途径
    Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
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