STAT5A ELISA 试剂盒
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- 抗原 See all STAT5A ELISA试剂盒
- STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))
- 抗原表位
- pTyr694, total
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Phospho-STAT5A (Tyr694) and Total STAT5A ELISA Kit. This assay semi-quantitatively measures STAT5A phosphorylated at Tyrosine-694 as well as total STAT5A in cell lysate samples.
- 样品类型
- Cell Culture Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- This ELISA kit recognizes Human STAT5A phosphorylated at site Tyrosine-694 as well as total STAT5A.
- 产品特性
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- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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Signal Transducer and Activator of Transcription 5A (STAT5A) ELISA Kit
STAT5A 适用: 人, 大鼠, 小鼠 pTyr694 Colorimetric Cell ELISA Cell Culture Cells
Signal Transducer and Activator of Transcription 5A (STAT5A) ELISA KitSTAT5A 适用: 人, 大鼠, 小鼠 pTyr Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Signal Transducer and Activator of Transcription 5A (STAT5A) ELISA KitSTAT5A 适用: 人, 大鼠, 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Signal Transducer and Activator of Transcription 5A (STAT5A) ELISA KitSTAT5A 适用: 人 pTyr694 Colorimetric Sandwich ELISA Cell Culture Lysate
Signal Transducer and Activator of Transcription 5A (STAT5A) ELISA KitSTAT5A 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all STAT5A ELISA试剂盒
- STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))
- 别名
- STAT5A (STAT5A 产品)
- 别名
- MGF ELISA Kit, STAT5 ELISA Kit, AA959963 ELISA Kit, Stat5 ELISA Kit, STAT5A/MGF ELISA Kit, STATA5 ELISA Kit, STAT5A ELISA Kit, stat5 ELISA Kit, stat5b ELISA Kit, signal transducer and activator of transcription 5A ELISA Kit, signal transducer and activator of transcription 5a ELISA Kit, STAT5A ELISA Kit, Stat5a ELISA Kit, stat5a ELISA Kit, LOC100348518 ELISA Kit, LOC100716811 ELISA Kit
- 基因ID
- 6776
- UniProt
- P42229
- 途径
- JAK/STAT Signaling, RTK signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation
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