Caspase 7 ELISA 试剂盒
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- 抗原 See all Caspase 7 (CASP7) ELISA试剂盒
- Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))
- 抗原表位
- Cleaved-Asp198
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适用
- 人, 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human/mouse cleaved CASP-7 (D198) ELISA Kit. This ELISA is for measuring cleaved CASP-7 (Asp-198) in human and mouse cell lysates.
- 样品类型
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- The antibody pair provided in this kit recognizes human/mouse cleaved-caspase-7 cleaved at site Aspartic Acid-198
- 产品特性
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- Rapidly measure cleaved protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-cleaved Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all Caspase 7 (CASP7) ELISA试剂盒
- Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))
- 别名
- Caspase-7 (CASP7 产品)
- 别名
- xCaspase-7 ELISA Kit, caspase-7 ELISA Kit, caspase7 ELISA Kit, cmh-1 ELISA Kit, ice-lap3 ELISA Kit, mch3 ELISA Kit, CASP7 ELISA Kit, AI314680 ELISA Kit, CMH-1 ELISA Kit, ICE-IAP3 ELISA Kit, Mch3 ELISA Kit, mCASP-7 ELISA Kit, CASP-7 ELISA Kit, ICE-LAP3 ELISA Kit, LICE2 ELISA Kit, MCH3 ELISA Kit, Caspase-7 ELISA Kit, MCH3/SCA-2 ELISA Kit, SCA-2 ELISA Kit, caspase 7 ELISA Kit, caspase-7 ELISA Kit, caspase 7 S homeolog ELISA Kit, CASP7 ELISA Kit, xcaspase-7 ELISA Kit, casp7 ELISA Kit, casp7.S ELISA Kit, Casp7 ELISA Kit
- UniProt
- P55210
- 途径
- Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
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