RPS6KA3 ELISA 试剂盒
RPS6KA3
适用: 人, 小鼠, 大鼠
pSer386
Colorimetric
Sandwich ELISA
Cell Culture Lysate
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- 抗原 See all RPS6KA3 ELISA试剂盒
- RPS6KA3 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3))
- 抗原表位
- pSer386, total
- 适用
- 人, 小鼠, 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human, Mouse and Rat Phospho-RSK2 (Ser386) and Total RSK2 ELISA Kit. This assay semi-quantitatively measures phophorylated RSK2 (Ser386) and Total RSK2 in lysate samples.
- 样品类型
- Cell Culture Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- This ELISA kit recognizes Human, Mouse and Rat RSK2 phosphorylated at site Serine-386 as well as total RSK2.
- 产品特性
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- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Top Product
- Discover our top product RPS6KA3 ELISA Kit
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all RPS6KA3 ELISA试剂盒
- RPS6KA3 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3))
- 别名
- RSK2 (RPS6KA3 产品)
- 别名
- HU-1 ELISA Kit, MAPKAPK1A ELISA Kit, RSK ELISA Kit, RSK1 ELISA Kit, CLS ELISA Kit, HU-3 ELISA Kit, ISPK-1 ELISA Kit, MAPKAPK1B ELISA Kit, MRX19 ELISA Kit, RSK2 ELISA Kit, S6K-alpha3 ELISA Kit, p90-RSK2 ELISA Kit, pp90RSK2 ELISA Kit, MPK-9 ELISA Kit, Rsk2 ELISA Kit, RGD1563860 ELISA Kit, id:ibd1103 ELISA Kit, rps6ka3 ELISA Kit, si:ch211-114c12.12 ELISA Kit, wu:fk86e02 ELISA Kit, zgc:56006 ELISA Kit, zgc:158386 ELISA Kit, ribosomal protein S6 kinase A1 ELISA Kit, ribosomal protein S6 kinase A3 ELISA Kit, ribosomal protein S6 kinase polypeptide 3 ELISA Kit, ribosomal protein S6 kinase a, polypeptide 3a ELISA Kit, ribosomal protein S6 kinase, polypeptide 3b ELISA Kit, RPS6KA1 ELISA Kit, RPS6KA3 ELISA Kit, Rps6ka3 ELISA Kit, rps6ka3a ELISA Kit, rps6ka3b ELISA Kit
- 基因ID
- 6197
- UniProt
- P51812, P18654, D3Z8E0
- 途径
- MAPK Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades
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