Phospholipase C gamma 2 ELISA 试剂盒
PLCG2
适用: 人, 小鼠
pTyr753
Colorimetric
Sandwich ELISA
Cell Lysate, Tissue Lysate
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- 抗原 See all Phospholipase C gamma 2 (PLCG2) ELISA试剂盒
- Phospholipase C gamma 2 (PLCG2)
- 抗原表位
- pTyr753, total
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适用
- 人, 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human and Mouse Phospho-PLCG2 (Tyr753) and Total PLCG2 ELISA Kit. This assay semi-quantitatively measures phophorylated PLCG2 (Tyr753) and Total PLCG2 in lysate samples.
- 样品类型
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- This ELISA kit recognizes Human and Mouse PLCG2 phosphorylated at site Tyrosine-753 as well as total PLCG2.
- 产品特性
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- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Top Product
- Discover our top product PLCG2 ELISA Kit
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Phospholipase C gamma 2 (PLCG2) ELISA Kit
PLCG2 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Phospholipase C gamma 2 (PLCG2) ELISA KitPLCG2 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all Phospholipase C gamma 2 (PLCG2) ELISA试剂盒
- Phospholipase C gamma 2 (PLCG2)
- 别名
- PLCG2 (PLCG2 产品)
- 别名
- PLCgamma2 ELISA Kit, Plcg-2 ELISA Kit, APLAID ELISA Kit, FCAS3 ELISA Kit, phospholipase C, gamma 2 ELISA Kit, phospholipase C gamma 2 ELISA Kit, phospholipase C, gamma 2 (phosphatidylinositol-specific) ELISA Kit, Plcg2 ELISA Kit, PLCG2 ELISA Kit
- 基因ID
- 5336
- UniProt
- P16885, Q8CIH5
- 途径
- RTK signaling, WNT signaling, Fc-epsilon Receptor Signaling Pathway, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades, VEGF Signaling, BCR Signaling
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