PAK2 ELISA 试剂盒
PAK2
适用: 人
pSer20
Colorimetric
Sandwich ELISA
Cell Lysate, Tissue Lysate
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- 抗原 See all PAK2 ELISA试剂盒
- PAK2 (P21-Activated Kinase 2 (PAK2))
- 抗原表位
- pSer20, total
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Phospho-PAK2 (Ser20) and Total PAK2 ELISA Kit. This assay semi-quantitatively measures phophorylated PAK2 (Ser20) and Total PAK2 in lysate samples.
- 样品类型
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- This ELISA kit recognizes Human PAK2 phosphorylated at site Serine-20 as well as total PAK2.
- 产品特性
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- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Top Product
- Discover our top product PAK2 ELISA Kit
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all PAK2 ELISA试剂盒
- PAK2 (P21-Activated Kinase 2 (PAK2))
- 别名
- PAK2 (PAK2 产品)
- 别名
- PAK65 ELISA Kit, PAKgamma ELISA Kit, gamma-PAK ELISA Kit, 5330420P17Rik ELISA Kit, A130002K10Rik ELISA Kit, AI836325 ELISA Kit, D16Ertd269e ELISA Kit, PAK-2 ELISA Kit, mKIAA4182 ELISA Kit, pak2 ELISA Kit, cb422 ELISA Kit, kinase ELISA Kit, zgc:91798 ELISA Kit, wu:fb71h01 ELISA Kit, si:dz198m22.1 ELISA Kit, pak65 ELISA Kit, pakgamma ELISA Kit, PAKI ELISA Kit, Pak3 ELISA Kit, CH73-159L23.1 ELISA Kit, wu:fc52b03 ELISA Kit, zgc:113857 ELISA Kit, p21 (RAC1) activated kinase 2 ELISA Kit, p21 protein (Cdc42/Rac)-activated kinase 2 ELISA Kit, p21 protein (Cdc42/Rac)-activated kinase 2a ELISA Kit, p21 protein (Cdc42/Rac)-activated kinase 2 S homeolog ELISA Kit, p21 protein (Cdc42/Rac)-activated kinase 2b ELISA Kit, PAK2 ELISA Kit, Pak2 ELISA Kit, pak2a ELISA Kit, pak2.S ELISA Kit, pak2b ELISA Kit
- 基因ID
- 5062
- UniProt
- Q13177
- 途径
- MAPK Pathway, RTK signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha
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