NGFR ELISA 试剂盒
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- 抗原 See all NGFR ELISA试剂盒
- NGFR (Nerve Growth Factor Receptor (NGFR))
- 抗原表位
- pTyr
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human Phosphotyrosine NGFR ELISA Kit. This assay semi-quantitatively measures phosphotyrosine NGFR in lysate samples.
- 样品类型
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-NGFR.
- 产品特性
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- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Biotinylated Anti-Phosphotyrosine Antibody
- Stop Solution
- Assay Diluent(s)
- Positive Control Sample
- Lysis Buffer
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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- Discover our best selling NGFR ELISA Kit
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- Discover our top product NGFR ELISA Kit
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Nerve Growth Factor Receptor (NGFR) ELISA Kit
NGFR 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Nerve Growth Factor Receptor (NGFR) ELISA KitNGFR 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Nerve Growth Factor Receptor (NGFR) ELISA KitNGFR 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Nerve Growth Factor Receptor (NGFR) ELISA KitNGFR 适用: 人 AA 29-250 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Nerve Growth Factor Receptor (NGFR) ELISA KitNGFR 适用: 人 Colorimetric Sandwich ELISA 80 pg/mL-20 ng/mL Cell Culture Supernatant, Plasma, Serum
Nerve Growth Factor Receptor (NGFR) ELISA KitNGFR 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all NGFR ELISA试剂盒
- NGFR (Nerve Growth Factor Receptor (NGFR))
- 别名
- NGFR (NGFR 产品)
- 别名
- ngfr ELISA Kit, wu:fb38c02 ELISA Kit, wu:fc10h01 ELISA Kit, CD271 ELISA Kit, Gp80-LNGFR ELISA Kit, TNFRSF16 ELISA Kit, p75(NTR) ELISA Kit, p75NTR ELISA Kit, LNGFR ELISA Kit, RNNGFRR ELISA Kit, Tnfrsf16 ELISA Kit, p75 ELISA Kit, NRH1b ELISA Kit, fullback ELISA Kit, nrh1 ELISA Kit, p75ntr ELISA Kit, tnfrsf16 ELISA Kit, p75NGFR ELISA Kit, nerve growth factor receptor ELISA Kit, neurotrophin receptor associated death domain ELISA Kit, nerve growth factor receptor S homeolog ELISA Kit, nerve growth factor receptor (TNFR superfamily, member 16) ELISA Kit, NGFR ELISA Kit, nradd ELISA Kit, Ngfr ELISA Kit, ngfr.S ELISA Kit
- 基因ID
- 5715
- UniProt
- O00233
- 途径
- NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding
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