MUSK ELISA 试剂盒
-
- 抗原 See all MUSK ELISA试剂盒
- MUSK (Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK))
- 抗原表位
- pTyr
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Phosphotyrosine MUSK ELISA Kit. This assay semi-quantitatively measures phosphotyrosine MUSK in lysate samples.
- 样品类型
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-MUSK.
- 产品特性
-
- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Biotinylated Anti-Phosphotyrosine Antibody
- Stop Solution
- Assay Diluent(s)
- Positive Control Sample
- Lysis Buffer
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Featured
- Discover our best selling MUSK ELISA Kit
- Top Product
- Discover our top product MUSK ELISA Kit
-
Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) ELISA Kit
MUSK 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) ELISA KitMUSK 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) ELISA KitMUSK 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) ELISA KitMUSK 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
-
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
-
- 抗原 See all MUSK ELISA试剂盒
- MUSK (Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK))
- 别名
- MuSK (MUSK 产品)
- 别名
- unp ELISA Kit, fj98e12 ELISA Kit, zgc:63467 ELISA Kit, wu:fj98e12 ELISA Kit, si:dz46m7.2 ELISA Kit, si:dz57f23.3 ELISA Kit, si:busm1-57f23.3 ELISA Kit, musk-A ELISA Kit, MuSK ELISA Kit, MUSK ELISA Kit, Mdk4 ELISA Kit, Mlk ELISA Kit, Nsk1 ELISA Kit, Nsk2 ELISA Kit, Nsk3 ELISA Kit, muscle ELISA Kit, muscle associated receptor tyrosine kinase ELISA Kit, muscle, skeletal, receptor tyrosine kinase ELISA Kit, muscle associated receptor tyrosine kinase S homeolog ELISA Kit, MUSK ELISA Kit, musk ELISA Kit, musk.S ELISA Kit, Musk ELISA Kit
- 基因ID
- 4593
- UniProt
- O15146
- 途径
- RTK signaling, Regulation of Muscle Cell Differentiation, Synaptic Membrane, Regulation of Cell Size, Skeletal Muscle Fiber Development
-
