LYN ELISA 试剂盒
LYN
适用: 人
pTyr
Colorimetric
Sandwich ELISA
Cell Lysate, Tissue Lysate
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- 抗原 See all LYN ELISA试剂盒
- LYN (V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN))
- 抗原表位
- pTyr
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human Phosphotyrosine LYN ELISA Kit. This assay semi-quantitatively measures phosphotyrosine LYN in lysate samples.
- 样品类型
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-LYN.
- 产品特性
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- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Biotinylated Anti-Phosphotyrosine Antibody
- Stop Solution
- Assay Diluent(s)
- Positive Control Sample
- Lysis Buffer
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Featured
- Discover our best selling LYN ELISA Kit
- Top Product
- Discover our top product LYN ELISA Kit
-
V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN) ELISA Kit
LYN 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
-
- 抗原 See all LYN ELISA试剂盒
- LYN (V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN))
- 别名
- LYN (LYN 产品)
- 别名
- JTK8 ELISA Kit, p53Lyn ELISA Kit, p56Lyn ELISA Kit, lyn ELISA Kit, LYN ELISA Kit, AA407514 ELISA Kit, Hck-2 ELISA Kit, jtk8 ELISA Kit, lyn-A ELISA Kit, CH73-38P6.3 ELISA Kit, zgc:92124 ELISA Kit, LYN proto-oncogene, Src family tyrosine kinase ELISA Kit, v-yes-1 Yamaguchi sarcoma viral related oncogene homolog ELISA Kit, tyrosine-protein kinase Lyn ELISA Kit, LYN proto-oncogene, Src family tyrosine kinase L homeolog ELISA Kit, LYN ELISA Kit, lyn ELISA Kit, Lyn ELISA Kit, LOC100593961 ELISA Kit, lyn.L ELISA Kit
- 基因ID
- 4067
- UniProt
- P07948
- 途径
- Fc-epsilon Receptor Signaling Pathway, Hormone Transport, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling, Integrin Complex, BCR Signaling
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