JNK ELISA 试剂盒
MAPK8
适用: 人, 小鼠, 大鼠
pThr183
Colorimetric
Sandwich ELISA
Cell Lysate, Tissue Lysate
(1 reference)-
- 抗原 See all JNK (MAPK8) ELISA试剂盒
- JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))
- 抗原表位
- pThr183, pTyr185, total
- 适用
- 人, 小鼠, 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human/Mouse/Rat Phospho-JNK (T183/Y185) and Total JNK ELISA Kit. This assay semi-quantitatively measures phophorylated JNK (Thr183/Tyr185) and Total JNK in lysate samples.
- 样品类型
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- The antibody pair provided in this kit recognizes Human/Mouse/Rat Phospho-JNK (pThr183/Tyr185) and total (pan) JNK.
- 产品特性
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- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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Mitogen-Activated Protein Kinase 8 (MAPK8) ELISA Kit
MAPK8 适用: 人, 小鼠, 大鼠 pThr183 Colorimetric Cell ELISA Cell Culture Cells
Mitogen-Activated Protein Kinase 8 (MAPK8) ELISA KitMAPK8 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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: "Protective effects of arjunolic acid against cardiac toxicity induced by oral sodium nitrite: effects on cytokine balance and apoptosis." in: Life sciences, Vol. 111, Issue 1-2, pp. 18-26, (2014) (PubMed).
-
: "Protective effects of arjunolic acid against cardiac toxicity induced by oral sodium nitrite: effects on cytokine balance and apoptosis." in: Life sciences, Vol. 111, Issue 1-2, pp. 18-26, (2014) (PubMed).
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- 抗原 See all JNK (MAPK8) ELISA试剂盒
- JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))
- 别名
- JNK (MAPK8 产品)
- 别名
- JNK ELISA Kit, JNK-46 ELISA Kit, JNK1 ELISA Kit, JNK1A2 ELISA Kit, JNK21B1/2 ELISA Kit, PRKM8 ELISA Kit, SAPK1 ELISA Kit, SAPK1c ELISA Kit, AI849689 ELISA Kit, Prkm8 ELISA Kit, BSK ELISA Kit, BSK/DJNK ELISA Kit, Bsk ELISA Kit, CG5680 ELISA Kit, D-JNK ELISA Kit, D-junk ELISA Kit, DBSK/JNK ELISA Kit, DJNK ELISA Kit, DJNK/bsk ELISA Kit, Dmel\\CG5680 ELISA Kit, JNK/SAPK ELISA Kit, Jnk ELISA Kit, Junk ELISA Kit, SAPKa ELISA Kit, c-Jun ELISA Kit, dJNK ELISA Kit, jnk ELISA Kit, T10F20.15 ELISA Kit, jnk1 ELISA Kit, sapk1 ELISA Kit, mapk8 ELISA Kit, zgc:112379 ELISA Kit, JNKb ELISA Kit, mapk8b ELISA Kit, mitogen-activated protein kinase 8 ELISA Kit, Stress-activated protein kinase jnk-1 ELISA Kit, basket ELISA Kit, Protein kinase superfamily protein ELISA Kit, mitogen-activated protein kinase 8 L homeolog ELISA Kit, mitogen-activated protein kinase 8b ELISA Kit, mitogen-activated protein kinase 8B-like ELISA Kit, MAPK8 ELISA Kit, jnk-1 ELISA Kit, Mapk8 ELISA Kit, bsk ELISA Kit, ATMPK8 ELISA Kit, mapk8.L ELISA Kit, mapk8b ELISA Kit, LOC109094263 ELISA Kit
- 基因ID
- 5599
- UniProt
- P45983
- 途径
- MAPK Pathway, WNT signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins
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