Insulin Receptor ELISA 试剂盒
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- 抗原 See all Insulin Receptor (INSR) ELISA试剂盒
- Insulin Receptor (INSR)
- 抗原表位
- pTyr1189, total
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Phospho-INSR (Y1189) and Total INSR ELISA Kit. This assay semi-quantitatively measures phophorylated INSR (Tyr1189) and Total INSR in lysate samples.
- 样品类型
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- The antibody pair provided in this kit recognizes Human INSR phosphorylated at site Tyrosine-1189 and total INSR.
- 产品特性
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- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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- Discover our best selling INSR ELISA Kit
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Insulin Receptor (INSR) ELISA Kit
INSR 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin Receptor (INSR) ELISA KitINSR 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin Receptor (INSR) ELISA KitINSR 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Insulin Receptor (INSR) ELISA KitINSR 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Insulin Receptor (INSR) ELISA KitSmall Sample INSR 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all Insulin Receptor (INSR) ELISA试剂盒
- Insulin Receptor (INSR)
- 别名
- Insulin R (INSR 产品)
- 别名
- CD220 ELISA Kit, HHF5 ELISA Kit, 4932439J01Rik ELISA Kit, D630014A15Rik ELISA Kit, IR ELISA Kit, IR-A ELISA Kit, IR-B ELISA Kit, 18402 ELISA Kit, CG18402 ELISA Kit, DIHR ELISA Kit, DILR ELISA Kit, DIR ELISA Kit, DIRH ELISA Kit, DIRbeta ELISA Kit, DInR ELISA Kit, DInr ELISA Kit, Dir-a ELISA Kit, Dir-b ELISA Kit, Dmel\\CG18402 ELISA Kit, INR ELISA Kit, INS ELISA Kit, Inr ELISA Kit, Inr-alpha ELISA Kit, Inr-beta ELISA Kit, InsR ELISA Kit, dINR ELISA Kit, dIR ELISA Kit, dIRH ELISA Kit, dInR ELISA Kit, dInr ELISA Kit, dInsR ELISA Kit, dinr ELISA Kit, dir ELISA Kit, er10 ELISA Kit, inr ELISA Kit, insulin/insulin-like growth factor receptor ELISA Kit, l(3)05545 ELISA Kit, l(3)93Dj ELISA Kit, l(3)er10 ELISA Kit, lnR ELISA Kit, ir-A ELISA Kit, CTK-1 ELISA Kit, ir ELISA Kit, INSR ELISA Kit, NV14476 ELISA Kit, cd220 ELISA Kit, hhf5 ELISA Kit, insulin receptor ELISA Kit, Insulin-like receptor ELISA Kit, insulin receptor L homeolog ELISA Kit, INSR ELISA Kit, Insr ELISA Kit, InR ELISA Kit, LOC100122567 ELISA Kit, LOC100451802 ELISA Kit, insr.L ELISA Kit
- 基因ID
- 3643
- UniProt
- P06213
- 途径
- NF-kappaB Signaling, RTK signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity
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