HDAC4 ELISA 试剂盒
HDAC4
适用: 人
pSer632
Colorimetric
Sandwich ELISA
Cell Lysate, Tissue Lysate
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- 抗原 See all HDAC4 ELISA试剂盒
- HDAC4 (Histone Deacetylase 4 (HDAC4))
- 抗原表位
- pSer632, total
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Phospho-HDAC4 (S632) and Total HDAC4 ELISA Kit. This assay semi-quantitatively measures phophorylated HDAC4 (Ser632) and Total HDAC4 in lysate samples.
- 样品类型
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- The antibody pair provided in this kit recognizes human HDAC4 phosphorylated at site Serine-632 and total HDAC4.
- 产品特性
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- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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- Discover our best selling HDAC4 ELISA Kit
- Top Product
- Discover our top product HDAC4 ELISA Kit
-
Histone Deacetylase 4 (HDAC4) ELISA Kit
HDAC4 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Tissue Homogenate
Histone Deacetylase 4 (HDAC4) ELISA KitHDAC4 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
-
- 抗原 See all HDAC4 ELISA试剂盒
- HDAC4 (Histone Deacetylase 4 (HDAC4))
- 别名
- HDAC4 (HDAC4 产品)
- 别名
- CG1770 ELISA Kit, DHDAC4 ELISA Kit, Dmel\\CG1770 ELISA Kit, GC1770 ELISA Kit, HDAC ELISA Kit, HDAC4a ELISA Kit, dHDAC4 ELISA Kit, dmHDA405 ELISA Kit, hdac4 ELISA Kit, dana_GLEANR_19966 ELISA Kit, DanaGF18710 ELISA Kit, GF18710 ELISA Kit, HDAC4 ELISA Kit, AHO3 ELISA Kit, BDMR ELISA Kit, HA6116 ELISA Kit, HD4 ELISA Kit, HDAC-4 ELISA Kit, HDAC-A ELISA Kit, HDACA ELISA Kit, 4932408F19Rik ELISA Kit, AI047285 ELISA Kit, wu:fa96d08 ELISA Kit, wu:fc56f08 ELISA Kit, zgc:152701 ELISA Kit, histone deacetylase 4 ELISA Kit, hypothetical protein ELISA Kit, Histone deacetylase 4 ELISA Kit, GF18710 gene product from transcript GF18710-RC ELISA Kit, PTRG_03699 ELISA Kit, PGTG_15512 ELISA Kit, HDAC4 ELISA Kit, Dana\GF18710 ELISA Kit, Hdac4 ELISA Kit, hdac4 ELISA Kit
- 基因ID
- 25698
- UniProt
- P56524
- 途径
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process
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