ERK1/2 ELISA 试剂盒
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- 抗原 See all ERK1/2 (MAPK1/3) ELISA试剂盒
- ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))
- 抗原表位
- pTyr
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human Phosphotyrosine ERK1/2 ELISA Kit. This assay semi-quantitatively measures phosphotyrosine ERK1/2 in lysate samples.
- 样品类型
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-ERK1/2.
- 产品特性
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- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Biotinylated Anti-Phosphotyrosine Antibody
- Stop Solution
- Assay Diluent(s)
- Positive Control Sample
- Lysis Buffer
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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- Discover our best selling MAPK1/3 ELISA Kit
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- Discover our top product MAPK1/3 ELISA Kit
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Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) ELISA Kit
MAPK1/3 适用: 人, 大鼠, 小鼠 pThr202 Colorimetric Cell ELISA Cell Culture Cells
Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) ELISA KitMAPK1/3 适用: 人, 大鼠, 小鼠 pThr202 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) ELISA KitMAPK1/3 适用: 人, 大鼠, 小鼠 pThr202 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) ELISA KitMAPK1/3 适用: 大鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) ELISA KitMAPK1/3 适用: 人 Phosphospecific Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
-
- 抗原 See all ERK1/2 (MAPK1/3) ELISA试剂盒
- ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))
- 别名
- ERK1///ERK2 (MAPK1/3 产品)
- 别名
- erk1/2 ELISA Kit, mitogen-activated protein kinase ELISA Kit, erk1/2 ELISA Kit
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