EPH Receptor B1 ELISA 试剂盒
EPHB1
适用: 人
pTyr
Colorimetric
Sandwich ELISA
Cell Lysate, Tissue Lysate
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- 抗原 See all EPH Receptor B1 (EPHB1) ELISA试剂盒
- EPH Receptor B1 (EPHB1)
- 抗原表位
- pTyr
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human Phosphotyrosine EphB1 ELISA Kit. This assay semi-quantitatively measures phosphotyrosine EphB1 in lysate samples.
- 样品类型
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-EphB1.
- 产品特性
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- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Biotinylated Anti-Phosphotyrosine Antibody
- Stop Solution
- Assay Diluent(s)
- Positive Control Sample
- Lysis Buffer
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
-
- 抗原 See all EPH Receptor B1 (EPHB1) ELISA试剂盒
- EPH Receptor B1 (EPHB1)
- 别名
- EphB1 (EPHB1 产品)
- 别名
- ELK ELISA Kit, EPHT2 ELISA Kit, Hek6 ELISA Kit, NET ELISA Kit, ephb1-a ELISA Kit, xek ELISA Kit, Xek ELISA Kit, MGC89790 ELISA Kit, EPHB1 ELISA Kit, CEK6 ELISA Kit, EK6 ELISA Kit, 9330129L11 ELISA Kit, AW488255 ELISA Kit, C130099E04Rik ELISA Kit, Cek6 ELISA Kit, ENSMUSG00000074119 ELISA Kit, Elk ELISA Kit, Elkh ELISA Kit, Net ELISA Kit, Ephb2 ELISA Kit, Erk ELISA Kit, elk ELISA Kit, EPH receptor B1 ELISA Kit, EPH receptor B1 S homeolog ELISA Kit, ephrin type-B receptor 1 ELISA Kit, Eph receptor B1 ELISA Kit, EPHB1 ELISA Kit, ephb1.S ELISA Kit, ephb1 ELISA Kit, LOC100467687 ELISA Kit, Ephb1 ELISA Kit
- 基因ID
- 2047
- UniProt
- P54762
- 途径
- RTK signaling
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