EPH Receptor A6 ELISA 试剂盒
Epha6
适用: 人
pTyr
Colorimetric
Sandwich ELISA
Cell Lysate, Tissue Lysate
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- 抗原 See all EPH Receptor A6 (Epha6) ELISA试剂盒
- EPH Receptor A6 (Epha6)
- 抗原表位
- pTyr
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human Phosphotyrosine EphA6 ELISA Kit. This assay semi-quantitatively measures phosphotyrosine EphA6 in lysate samples.
- 样品类型
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-EphA6.
- 产品特性
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- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Biotinylated Anti-Phosphotyrosine Antibody
- Stop Solution
- Assay Diluent(s)
- Positive Control Sample
- Lysis Buffer
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all EPH Receptor A6 (Epha6) ELISA试剂盒
- EPH Receptor A6 (Epha6)
- 别名
- EphA6 (Epha6 产品)
- 别名
- EPHA6 ELISA Kit, Ehk2 ELISA Kit, Hek12 ELISA Kit, m-ehk2 ELISA Kit, EHK-2 ELISA Kit, EHK2 ELISA Kit, EK12 ELISA Kit, EPA6 ELISA Kit, HEK12 ELISA Kit, PRO57066 ELISA Kit, EPH receptor A6 ELISA Kit, Eph receptor A6 ELISA Kit, EPHA6 ELISA Kit, epha6 ELISA Kit, Epha6 ELISA Kit
- 基因ID
- 285220
- UniProt
- Q9UF33
- 途径
- RTK signaling
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