DDR2 ELISA 试剂盒
DDR2
适用: 人
pTyr740
Colorimetric
Sandwich ELISA
Cell Lysate, Tissue Lysate
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- 抗原 See all DDR2 ELISA试剂盒
- DDR2 (Discoidin Domain Receptor tyrosine Kinase 2 (DDR2))
- 抗原表位
- pTyr740, total
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Phospho-DDR2 (Tyr740) and Total DDR2 ELISA Kit. This assay semi-quantitatively measures phophorylated DDR2 (Tyr740) and Total DDR2 in lysate samples.
- 样品类型
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- This ELISA kit recognizes Human DDR2 phosphorylated at site Tyrosine-740 as well as total DDR2.
- 产品特性
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- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Featured
- Discover our best selling DDR2 ELISA Kit
- Top Product
- Discover our top product DDR2 ELISA Kit
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Discoidin Domain Receptor tyrosine Kinase 2 (DDR2) ELISA Kit
DDR2 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Discoidin Domain Receptor tyrosine Kinase 2 (DDR2) ELISA KitDDR2 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
-
- 抗原 See all DDR2 ELISA试剂盒
- DDR2 (Discoidin Domain Receptor tyrosine Kinase 2 (DDR2))
- 别名
- DDR2 (DDR2 产品)
- 别名
- MIG20a ELISA Kit, NTRKR3 ELISA Kit, TKT ELISA Kit, TYRO10 ELISA Kit, AW495251 ELISA Kit, Ntrkr3 ELISA Kit, tyro10 ELISA Kit, DDR2 ELISA Kit, Tyro10 ELISA Kit, ddr2 ELISA Kit, si:ch211-193c2.1 ELISA Kit, discoidin domain receptor tyrosine kinase 2 ELISA Kit, discoidin domain receptor family, member 2 ELISA Kit, discoidin domain receptor family, member 2, like ELISA Kit, DDR2 ELISA Kit, Ddr2 ELISA Kit, ddr2l ELISA Kit
- 基因ID
- 4921
- UniProt
- Q16832
- 途径
- RTK signaling
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