CHEK2 ELISA 试剂盒
CHEK2
适用: 人
pThr68
Colorimetric
Sandwich ELISA
Cell Lysate, Tissue Lysate
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- 抗原 See all CHEK2 ELISA试剂盒
- CHEK2 (Checkpoint Kinase 2 (CHEK2))
- 抗原表位
- pThr68
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Phospho-Chk2 (T68) ELISA Kit. This assay semi-quantitatively measures phophorylated Chk2 (Thr68) in lysate samples.
- 样品类型
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- The antibody pair provided in this kit recognizes Human CHK2 phosphorylated at site Threonine-68.
- 产品特性
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- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Biotinylated Anti-Phosphotyrosine Antibody
- Stop Solution
- Assay Diluent(s)
- Positive Control Sample
- Lysis Buffer
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Top Product
- Discover our top product CHEK2 ELISA Kit
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all CHEK2 ELISA试剂盒
- CHEK2 (Checkpoint Kinase 2 (CHEK2))
- 别名
- CHK2 (CHEK2 产品)
- 别名
- CDS1 ELISA Kit, CHK2 ELISA Kit, HuCds1 ELISA Kit, LFS2 ELISA Kit, PP1425 ELISA Kit, RAD53 ELISA Kit, hCds1 ELISA Kit, fa66f08 ELISA Kit, wu:fa66f08 ELISA Kit, zgc:55865 ELISA Kit, Cds1 ELISA Kit, HUCDS1 ELISA Kit, Rad53 ELISA Kit, Chk2 ELISA Kit, cds1 ELISA Kit, chek2 ELISA Kit, chk2 ELISA Kit, hucds1 ELISA Kit, lfs2 ELISA Kit, pp1425 ELISA Kit, rad53 ELISA Kit, checkpoint kinase 2 ELISA Kit, serine/threonine-protein kinase chk2 ELISA Kit, checkpoint kinase 2 L homeolog ELISA Kit, CHEK2 ELISA Kit, chek2 ELISA Kit, Chek2 ELISA Kit, MCYG_07308 ELISA Kit, chek2.L ELISA Kit
- 基因ID
- 11200
- UniProt
- O96017
- 途径
- p53 Pathway, Apoptosis, Cell Division Cycle
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