ATF2 ELISA 试剂盒
-
- 抗原 See all ATF2 ELISA试剂盒
- ATF2 (Activating Transcription Factor 2 (ATF2))
- 抗原表位
- pThr69, pThr71, total
-
适用
- 人, 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human and Mouse Phospho-ATF2 (Thr69/71) and Total ATF2 ELISA Kit. This assay semi-quantitatively measures phophorylated ATF2 (Thr69/71) and Total ATF2 in lysate samples.
- 样品类型
- Cell Culture Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- This ELISA kit recognizes Human and Mouse ATF2 phosphorylated at site Threonine-69/71 as well as total ATF2.
- 产品特性
-
- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
-
- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Featured
- Discover our best selling ATF2 ELISA Kit
- Top Product
- Discover our top product ATF2 ELISA Kit
-
Activating Transcription Factor 2 (ATF2) ELISA Kit
ATF2 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Activating Transcription Factor 2 (ATF2) ELISA KitATF2 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Activating Transcription Factor 2 (ATF2) ELISA KitATF2 适用: 人, 大鼠, 小鼠 pSer386 Colorimetric Sandwich ELISA Cell Culture Lysate
-
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
-
- 抗原 See all ATF2 ELISA试剂盒
- ATF2 (Activating Transcription Factor 2 (ATF2))
- 别名
- ATF2 (ATF2 产品)
- 别名
- CRE-BP1 ELISA Kit, CREB2 ELISA Kit, HB16 ELISA Kit, TREB7 ELISA Kit, CG12850 ELISA Kit, CG3749 ELISA Kit, dATF-2 ELISA Kit, DmelCG30420 ELISA Kit, CG30420 ELISA Kit, CHN1 ELISA Kit, Atf-2 ELISA Kit, CRE-BP ELISA Kit, Creb2 ELISA Kit, D130078H02Rik ELISA Kit, D18875 ELISA Kit, Tg(Gzma-Klra1)7Wum ELISA Kit, mXBP ELISA Kit, atf2-A ELISA Kit, atf2l ELISA Kit, activating transcription factor 2 ELISA Kit, Activating transcription factor-2 ELISA Kit, activating transcription factor 2 S homeolog ELISA Kit, ATF2 ELISA Kit, Atf-2 ELISA Kit, Atf2 ELISA Kit, atf2.S ELISA Kit, atf2 ELISA Kit
- 基因ID
- 1386
- UniProt
- P15336, P16951
- 途径
- MAPK Pathway, RTK signaling, Thyroid Hormone Synthesis, Activation of Innate immune Response, Chromatin Binding, Myometrial Relaxation and Contraction, Synaptic Membrane, Tube Formation, Toll-Like Receptors Cascades
-
