电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

eIF4EBP1 ELISA 试剂盒

EIF4EBP1 适用: 小鼠, 人 pThr36 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
产品编号 ABIN4885951
发货至: 中国
  • 抗原 See all eIF4EBP1 (EIF4EBP1) ELISA试剂盒
    eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))
    抗原表位
    pThr36
    适用
    • 7
    • 6
    • 5
    • 1
    小鼠, 人
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human/Mouse Phospho-4E-BP1 (T36) ELISA Kit. This assay semi-quantitatively measures phophorylated 4E-BP1 (Thr36) in lysate samples.
    样品类型
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    特异性
    The antibody pair provided in this kit recognizes human and mouse 4E-BP1 phosphorylated at site Threonine-36.
    产品特性
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    试剂未包括
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product EIF4EBP1 ELISA Kit
  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    有效期
    6 months
  • 抗原 See all eIF4EBP1 (EIF4EBP1) ELISA试剂盒
    eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))
    别名
    4E-BP1 (EIF4EBP1 产品)
    别名
    4E-BP1 ELISA Kit, 4EBP1 ELISA Kit, BP-1 ELISA Kit, PHAS-I ELISA Kit, 4e-bp1 ELISA Kit, AA959816 ELISA Kit, 153432_at ELISA Kit, 43-BP ELISA Kit, 4E-BP ELISA Kit, 4EBP ELISA Kit, 4e-bp ELISA Kit, 4eBP ELISA Kit, 4ebp ELISA Kit, BcDNA.HL08053 ELISA Kit, BcDNA:HL08053 ELISA Kit, CG8846 ELISA Kit, CT8705 ELISA Kit, Dm 4E-BP ELISA Kit, Dmel\\CG8846 ELISA Kit, Phas1 ELISA Kit, Thor/EIF4EBP ELISA Kit, anon-WO0172774.158 ELISA Kit, d4E-BP ELISA Kit, d4E-BP1 ELISA Kit, d4EBP ELISA Kit, d4e-bp ELISA Kit, d4eBP ELISA Kit, dthor ELISA Kit, eIF4E-BP ELISA Kit, l(2)06270 ELISA Kit, l(2)k07736 ELISA Kit, l(2)k13517 ELISA Kit, pp20 ELISA Kit, thor ELISA Kit, EIF4EBP1 ELISA Kit, fc04h09 ELISA Kit, wu:fc04h09 ELISA Kit, zgc:64137 ELISA Kit, eukaryotic translation initiation factor 4E binding protein 1 ELISA Kit, CG8846 gene product from transcript CG8846-RA ELISA Kit, EIF4EBP1 ELISA Kit, Eif4ebp1 ELISA Kit, Thor ELISA Kit, eif4ebp1 ELISA Kit
    基因ID
    1978
    UniProt
    Q13541
    途径
    MAPK Pathway, PI3K-Akt Signaling, RTK signaling, AMPK Signaling, Regulation of Cell Size, BCR Signaling
You are here:
客服