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IGFBP5 ELISA 试剂盒
IGFBP5
适用: 大鼠, 小鼠
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Plasma, Serum
IGFBP5ELISA Kit详情
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IGFBP5
(Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))
适用
All reactivities for IGFBP5 ELISA试剂盒
大鼠, 小鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
原理
Custom Rat IGFBP-5 SpeedELISA Kit.
品牌
SpeedELISA
样品类型
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
产品特性
Compressed workflow with only one wash step and three incubation steps 3 hours total processing time Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Streptavidin-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Capture Antibody HRP Conjugated Detection Antibody TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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样本量
100 μL
实验时间
3 h
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 70 μL of the Antibody Cocktail to each well. Incubate 10 minutes at RT. Add 50 μL of sample or standard to each well. Incubate 2 h at RT. Wash Wells Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
有效期
6 months
IGFBP5目标详情
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See all IGFBP5 ELISA试剂盒
IGFBP5
(Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))
别名
IGFBP-5 (IGFBP5 产品 )
别名
IBP5 ELISA Kit, AI256729 ELISA Kit, AW208790 ELISA Kit, IGFBP-5 ELISA Kit, IGFBP-5P ELISA Kit, IGF-BP5 ELISA Kit, igfbp5 ELISA Kit, ibp5 ELISA Kit, IBP-5 ELISA Kit, xIGFBP-5 ELISA Kit, IGFBP5 ELISA Kit, igfbp5b ELISA Kit, zgc:165472 ELISA Kit, insulin like growth factor binding protein 5 ELISA Kit, insulin-like growth factor binding protein 5 ELISA Kit, insulin like growth factor binding protein 5 L homeolog ELISA Kit, insulin-like growth factor binding protein 5b ELISA Kit, insulin-like growth factor-binding protein 5 ELISA Kit, insulin-like growth factor binding protein 5a ELISA Kit, IGFBP5 ELISA Kit, Igfbp5 ELISA Kit, igfbp5.L ELISA Kit, igfbp5b ELISA Kit, igfbp5 ELISA Kit, LOC100194500 ELISA Kit, igfbp5a ELISA Kit
基因ID
25285
UniProt
P24594
途径
WNT signaling , Carbohydrate Homeostasis , Myometrial Relaxation and Contraction , Regulation of Carbohydrate Metabolic Process , Autophagy , Smooth Muscle Cell Migration , Growth Factor Binding
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