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XIAP ELISA 试剂盒
XIAP
适用: 大鼠
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
XIAPELISA Kit详情
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XIAP
(X-Linked Inhibitor of Apoptosis (XIAP))
适用
All reactivities for XIAP ELISA试剂盒
大鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
原理
Custom Rat XIAP ELISA Kit.
样品类型
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
特异性
The antibody pair provided in this kit recognizes Rat XIAP.
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
XIAP目标详情
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抗原
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XIAP
(X-Linked Inhibitor of Apoptosis (XIAP))
别名
XIAP (XIAP 产品 )
别名
API3 ELISA Kit, BIRC4 ELISA Kit, IAP-3 ELISA Kit, ILP1 ELISA Kit, MIHA ELISA Kit, XLP2 ELISA Kit, hIAP-3 ELISA Kit, hIAP3 ELISA Kit, api3 ELISA Kit, birc4 ELISA Kit, ilp1 ELISA Kit, miha ELISA Kit, xlp2 ELISA Kit, xxiap ELISA Kit, 1110015C02Rik ELISA Kit, Aipa ELISA Kit, Api3 ELISA Kit, Birc4 ELISA Kit, IAP3 ELISA Kit, ILP-1 ELISA Kit, mXIAP ELISA Kit, riap3 ELISA Kit, fb73d01 ELISA Kit, wu:fb73d01 ELISA Kit, zgc:91873 ELISA Kit, BIRC8 ELISA Kit, X-linked inhibitor of apoptosis ELISA Kit, X-linked inhibitor of apoptosis L homeolog ELISA Kit, XIAP ELISA Kit, xiap ELISA Kit, xiap.L ELISA Kit, Xiap ELISA Kit
基因ID
63879
UniProt
Q9R0I6
途径
Apoptosis , Caspase Cascade in Apoptosis , Transition Metal Ion Homeostasis
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