Clusterin ELISA 试剂盒
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- 抗原 See all Clusterin (CLU) ELISA试剂盒
- Clusterin (CLU)
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Mouse Clusterin (Apolipoprotein J) ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- The antibody pair provided in this kit recognizes mouse Clusterin.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all Clusterin (CLU) ELISA试剂盒
- Clusterin (CLU)
- 别名
- Clusterin (CLU 产品)
- 别名
- LOC100136120 ELISA Kit, clu ELISA Kit, MGC53055 ELISA Kit, zgc:73352 ELISA Kit, xclu ELISA Kit, MGC89163 ELISA Kit, CLU ELISA Kit, APO-J ELISA Kit, APOJ ELISA Kit, CLI ELISA Kit, CLU1 ELISA Kit, CLU2 ELISA Kit, KUB1 ELISA Kit, NA1/NA2 ELISA Kit, SGP-2 ELISA Kit, SGP2 ELISA Kit, SP-40 ELISA Kit, TRPM-2 ELISA Kit, TRPM2 ELISA Kit, DAG ELISA Kit, RATTRPM2B ELISA Kit, SP40 ELISA Kit, TRPM2B ELISA Kit, Trpm2 ELISA Kit, Trpmb ELISA Kit, AI893575 ELISA Kit, ApoJ ELISA Kit, Cli ELISA Kit, D14Ucla3 ELISA Kit, Sgp-2 ELISA Kit, Sgp2 ELISA Kit, Sugp-2 ELISA Kit, GP80 ELISA Kit, gpIII ELISA Kit, 40 ELISA Kit, clusterin-1 ELISA Kit, clusterin L homeolog ELISA Kit, clusterin ELISA Kit, Clusterin ELISA Kit, LOC100136120 ELISA Kit, clu.L ELISA Kit, clu ELISA Kit, CLU ELISA Kit, clus ELISA Kit, Clu ELISA Kit, LOC101113728 ELISA Kit
- 基因ID
- 12759
- UniProt
- Q06890
- 途径
- Apoptosis, Negative Regulation of intrinsic apoptotic Signaling
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